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EC number: 290-971-9 | CAS number: 90294-83-0
The toxicity of the test item to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 and Council Regulation (EC) No. 266/2016/Method C.3 from 2018-06-11 to 2018-06-14 at the test facility. The aim of the study was the determination of the effects on growth rate and yield over a period of 72 hours.
The test item is a blue powder and has a water solubility of > 100 mg/L. The study was conducted under static conditions with an initial cell density of 5523 cells/mL. A stock solution and four concentration levels were tested in a geometrical series with a dilution factor of √10: 1.00 - 3.162 - 10.0 - 31.62 - 100 mg/L.
Three replicates were tested for each concentration level and six replicates for the control. The environmental conditions were within the acceptable limits.
The concentrations of the test item were analytically verified via photometric analysis at a wavelength of 662 nm in the fresh media at the start of the exposure (0 hours) and in the old media at the end of the exposure (72 hours) in all concentration levels and in the control.
The measured concentrations of the test item in fresh media at the start of the exposure (0 hours) were in the range of 94 to 105% of the nominal test item concentrations. At the end of the exposure period (72 hours), the measured test item concentrations in the old media were in the range of 96 to 107% of the test item concentrations.
Since the measured concentration of the test item did remain stable within ± 20% of the nominal concentration throughout the exposure period, the nominal concentrations were used for evaluation.
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