Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 96 hours. Measured test concentrations were determined
from samples of control and test solution. The samples of the test medium were analysed via LCMS/
MS-method.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenzuchtbetrieb Störk, 88348 Bad Saulgau, Germany
- Length at study initiation (length definition, mean, range and SD): 5.39 cm ± 0.4 cm (Mean ± SD)
- Weight at study initiation (mean and range, SD): 1.57 ± 0.29 g (Mean ± SD).


ACCLIMATION
- Acclimation period:12 days
- Acclimation conditions (same as test or not): yes
-

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
Total hardness as CaCO3 (mg/L): 250
Test temperature:
13.1 - 13.5 °C
pH:
7.1 - 7.9
Dissolved oxygen:
85-100%
Nominal and measured concentrations:
measured concentrations remained
within ± 20 % (85 – 120 %) of the nominal concentration

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
11.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes