Registration Dossier
Registration Dossier
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EC number: 813-120-0 | CAS number: 1262967-45-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 January - 10 September 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study was conducted in accordance with International guidelines and in accordance with GLP. All guideline validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: N/A
- Specific activity: N/A
- Locations of the label: N/A
- Expiration date of radiochemical substance: N/A
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature in a closed container.
- Stability under test conditions: Asumed stable.
- Solubility and stability of the test substance in the solvent/vehicle: Acetone stock solution prepared at 25 mg/mL
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: N/A
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: N/A
- Preliminary purification step (if any): N/A
- Final dilution of a dissolved solid, stock liquid or gel: N/A
- Final preparation of a solid: N/A
FORM AS APPLIED IN THE TEST (if different from that of starting material): Solubilised in an acetone stock solution which further diluted with acetone and spiked into dilution water. Co-solvent was maintained <0.1 %v/v in the test media.
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable): N/A
OTHER SPECIFICS: No - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.65, 1.3 and 2.6 mg/L nominal
- Sampling method: Aliquots of the fresh and expired solutions from Day 7, 13, 19 and 28 were taken for analysis. Samples were diluted 1:1 with acetonitrile. Samples from the 1.3 and 2.6 mg/L groups were further diluted 2.5 and 5 times with 50 % acetonitrile (aq.) and then filtered (0.22 µm) in preparation for LC-MS analysis.
- Sample storage conditions before analysis: Not reported - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 25 mg/mL acetone stock solution was prepared by dispensing 0.25917 - 0.26192 gtest item and dissolving in 10 mL acetone. Aliquots of the stock were diluted (in acetone) to prepare a further 2 stock solutions with a concentration of 6.25 and 12.5 mg/mL. 0.2 mL of each stock was dispensed into 2 L of test medium to prepare the treatment solutions. The solutions were stirred for 2 h.
- Eluate: Demineralised water which was groundwater filtered with activated charcoal and softened with "special techniques".
- Controls: Acetone solvent control run in parallel with treatment solutions/ test groups.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 0.01 % v/v
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: Danio rerio
- Source: Not reported
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): Not reported
- Method of collection of fertilised eggs: Not reported
- Subsequent handling of eggs: 60 fertilised eggs were used for each concentration and divided equally into 2 replicate chambers.
POST-HATCH FEEDING
- Start date: Not reported
- Type/source of feed: Yolk and fish feed powder was fed to newly hatched fry and 48 h old brine shrimp nauplii and fish feed powder for juveniles.
- Amount given: Not reported
- Frequency of feeding: ad libitum - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Hardness:
- 96 - 104 (units not reported)
- Test temperature:
- 24.2 - 25.6 ºC
- pH:
- 7.87 - 8.16
- Dissolved oxygen:
- 72.8 - 120.4 % ASV
- Salinity:
- N/A
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal: 0.65, 1.3 and 2.6 mg/L
Geometric mean measured: 0.537, 1.24 and 2.25 mg/L - Details on test conditions:
- TEST SYSTEM
- Emybro cups (if used, type/material, size, fill volume): 250 mL and 1 L glass beakers filled to 200 mL
- Test vessel: 250 mL and 1 L glass beakers filled to 200 mL
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 mL and 1 L glass beakers filled to 200 mL
- Aeration: Not reported
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A, semi-static test
- Renewal rate of test solution (frequency/flow rate): Test solutions renewed every 24 h
- No. of fertilized eggs/embryos per vessel: 30
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 0
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Demineralised water which was groundwater filtered with activated charcoal and softened by "special techniques".
- Total organic carbon: Not reproted
- Particulate matter: Not reproted
- Metals: Not reproted
- Pesticides: Not reproted
- Chlorine: Not reproted
- Alkalinity: Not reproted
- Ca/mg ratio: Not reproted
- Salinity: Not reproted
- Culture medium different from test medium: No
- Intervals of water quality measurement: Total hardness and pH were measured in all vessels at the beginning and end of the test. DO and temperature were measured twice weekly with temperature also continuously monitored in one vessel.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12: 12 light: dark
- Light intensity: Not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Embryonic development - verified as precisely as possible.
Hatching and survival - observed once daily and numbers recorded (including eggs, embryos, larvae and juvenile fish).
Abnormal appearance - recorded at adequate time intervals.
Length and weight - recorded at the end of the test.
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: No
- Results used to determine the conditions for the definitive study: No
POST-HATCH DETAILS
- Begin of post-hatch period: Day 4 = Day 0 post-hatch i.e. 90 % control embryos (and in all treatment groups) hatched by Day 4
- No. of hatched eggs (alevins)/treatment released to the test chamber: N/A embryo cups not utilised.
- Release of alevins from incubation cups to test chamber on day no.: N/A, embryo cups not utilised.
FERTILIZATION SUCCESS STUDY
- Number of eggs used: 60 eggs used per concentration
- Removal of eggs to check the embryonic development on day no.: Not conducted - Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.25 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and sub-lethal effects
- Details on results:
- - Mortality/survival at embryo, larval, juvenile, and adult stages: No significant differences were noted between the control and treatment groups.
- Days to hatch or time to release of young: No significant differences were noted between the control and treatment groups.
- Numbers hatched, Numbers of offspring produced, or Number of offspring per live female per day: No significant differences were noted between the control and treatment groups.
- Number of fish in swim-up stage at one or more time periods (e.g., day x1, x2): Not reported
- Observations on body length and weight of young and/or exposed parents at one or more time periods: No significant differences were noted between the control and treatment groups.
- Number of healthy fish at end of test: No significant differences were noted between the control and treatment groups.
- Type of and number with morphological abnormalities: No significant differences were noted between the control and treatment groups.
- Type of and number with behavioural abnormalities:No significant differences were noted between the control and treatment groups.
- Type and number of developmental effects: No significant differences were noted between the control and treatment groups.
- Type and magnitude of hormonal changes: Not determined
- Other biological observations: None
- Effect concentrations exceeding solubility of substance in test medium: No
- Incidents in the course of the test which might have influenced the results: No - Reported statistics and error estimates:
- The following parameters were available for statistical analysis; cummulative mortality, healthy number of larvae at the end of the test, time to start of hatching and end of hatching (90 % hatched), numbers of larvae hatching each day, length and weight of survivors at test end.
Analysis of variance procedures were used for calculating the variations. Multiple comparisons between the results at the individual concentrations and those for the controls were made. - Validity criteria fulfilled:
- yes
- Conclusions:
- There were no significant differences between the control vessels and the treatment groups. The following parameters were observed; mortality and sub-lethal effects (including: number of larvae hatched; time to hatching; number of healthy larvae; length and weight of larvae). The NOEC was therefore estimated as 2.25 mg/L, the highest concentration tested.
- Executive summary:
OECD 210 (2013) - The 28–day chronic toxicity of Tetraesters of pentaerythritol with 2-methylpropanoic acid and 3,5,5-trimethyl-hexanoic acid to early life stage of zebra fish (Danio rerio) was studied under semi-static conditions. Fertilized eggs/embryos, 30 per replicate vessel, of zebra fish were exposed to control, solvent control (acetone, 0.01 % v/v), and test chemical geometric mean measured concentrations of 0.537, 1.24 and 2.25 mg/L. The test system was maintained at 24.2 – 25.6 ºC and a pH of 7.87 to 8.16.
There were no treatment related effects observed during the test.
The 28-day NOEC value, based on mortality/sublethal effects, was 2.25 mg/L, the highest concentration tested.
This toxicity study is classified as acceptable and satisfies the guideline requirement for early life toxicity study with fish.
Reference
Table 1 Mean measured concentrations
Nominal concentration (mg/L) |
Geometric mean measured concentration (mg/L) |
|||||
Component 1 |
Component 2 |
Component 3 |
Component 4 |
Test item |
% of nominal |
|
Control |
nd |
nd |
nd |
nd |
nd |
nd |
Solvent control |
nd |
nd |
nd |
nd |
nd |
nd |
0.65 |
0.0582 |
0.171 |
0.202 |
0.105 |
0.537 |
82.5 |
1.3 |
0.116 |
0.402 |
0.476 |
0.249 |
1.24 |
95.4 |
2.6 |
0.226 |
0.721 |
0.868 |
0.430 |
2.25 |
86.3 |
nd: not detected
Table 2 Number of larvae hatched each day
Nominal concentration (mg/L) |
Mean measured concentrations (mg/L) |
Rep. |
Initial egg number |
Day 1 pre-hatch |
Day 2 pre-hatch |
Day 3 pre-hatch |
Day 0 post-hatch |
Day 1 post-hatch |
Control |
nd |
1 |
30 |
0 |
3 |
13 |
28 |
30 |
2 |
30 |
0 |
1 |
13 |
29 |
30 |
||
Solvent control |
nd |
1 |
30 |
0 |
1 |
19 |
29 |
30 |
2 |
30 |
0 |
2 |
10 |
29 |
30 |
||
0.65 |
0.537 |
1 |
30 |
0 |
1 |
14 |
30 |
30 |
2 |
30 |
0 |
1 |
15 |
30 |
30 |
||
1.3 |
1.24 |
1 |
30 |
0 |
1 |
21 |
30 |
30 |
2 |
30 |
0 |
1 |
24 |
28 |
30 |
||
2.6 |
2.25 |
1 |
30 |
0 |
2 |
15 |
30 |
30 |
2 |
30 |
0 |
1 |
15 |
30 |
30 |
nd: not detected
Table 3 Cummulative mortality
Test phase |
Nominal concentration (mg/L) |
Mortality and symptoms |
||||||||||||||||||||||
Control |
Solvent control |
0.65 |
1.3 |
2.6 |
||||||||||||||||||||
Measured concentration (mg/L) |
nd |
nd |
0.537 |
1.24 |
2.25 |
|||||||||||||||||||
Initial numbers |
1 |
2 |
1 |
2 |
1 |
2 |
1 |
2 |
1 |
2 |
||||||||||||||
Day 1 pre-hatch |
30 |
5 |
A |
6 |
A |
7 |
D |
5 |
A |
6 |
A |
5 |
A |
6 |
D |
6 |
D |
7 |
A |
9 |
A |
|||
Day 2 pre-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
6 |
D |
6 |
D |
7 |
A |
9 |
A |
|||
Day 3 pre-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
D |
6 |
D |
7 |
A |
9 |
A |
|||
Day 0 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 1 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 2 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 3 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 4 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 5 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 6 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 7 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 8 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 9 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 10 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 11 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 12 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 13 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 14 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 15 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 16 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 17 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 18 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 19 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 20 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 21 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 22 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 23 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 24 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 25 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 26 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 27 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 28 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 29 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Day 30 post-hatch |
30 |
5 |
A |
6 |
A |
7 |
A |
5 |
A |
6 |
A |
6 |
A |
7 |
A |
6 |
A |
7 |
A |
9 |
A |
|||
Survived fish |
30 |
25 |
24 |
23 |
25 |
24 |
24 |
23 |
24 |
23 |
21 |
|||||||||||||
Mortality (%) |
30 |
16.7 |
20.0 |
23.3 |
16.7 |
20.0 |
20.0 |
23.3 |
20.0 |
23.3 |
30.0 |
|||||||||||||
Survival rate (%) |
30 |
83.3 |
80.0 |
76.7 |
83.3 |
80.0 |
80.0 |
76.7 |
80.0 |
76.7 |
70.0 |
|||||||||||||
P0.05(2-side) |
- |
- |
0.795 |
0.500 |
0.500 |
0.126 |
||||||||||||||||||
Table 4 Weight of surviving animals at the end of the test
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Replicate |
Surviving numbers |
Total body weight (mg) |
Mean body weight (mg) |
Mean ± SD (mg) |
P0.05 (2-side) |
Control |
nd |
1 |
25 |
58.3 |
2.33 |
2.37 ± 0.057 |
- |
2 |
24 |
57.9 |
2.41 |
||||
Solvent control |
nd |
1 |
23 |
53.4 |
2.32 |
2.34 ± 0.021 |
0.39 |
2 |
25 |
58.8 |
2.35 |
||||
0.65 |
0.536 |
1 |
24 |
57.4 |
2.39 |
2.37 ± 0.032 |
0.96 |
2 |
24 |
56.3 |
2.35 |
||||
1.3 |
1.24 |
1 |
23 |
51.7 |
2.25 |
2.23 ± 0.022 |
0.24 |
2 |
24 |
53.2 |
2.22 |
||||
2.6 |
2.25 |
1 |
23 |
48.8 |
2.12 |
2.14 ± 0.025 |
0.061 |
2 |
21 |
45.3 |
2.16 |
Description of key information
Mortality and sub-lethal effects: NOEC = 2.25 mg/L; OECD 210; Hairong, Y. (2013)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2.25 mg/L
Additional information
OECD 210 (2013) - The 28–day chronic toxicity of Tetraesters of pentaerythritol with 2-methylpropanoic acid and 3,5,5-trimethyl-hexanoic acid to early life stage of zebra fish (Danio rerio) was studied under semi-static conditions. Fertilized eggs/embryos, 30 per replicate vessel, of zebra fish were exposed to control, solvent control (acetone, 0.01 % v/v), and test chemical geometric mean measured concentrations of 0.537, 1.24 and 2.25 mg/L. The test system was maintained at 24.2 – 25.6 ºC and a pH of 7.87 to 8.16.
There were no treatment related effects observed during the test.
The 28-day NOEC value, based on mortality/sublethal effects, was 2.25 mg/L, the highest concentration tested.
This toxicity study is classified as acceptable and satisfies the guideline requirement for early life toxicity study with fish.
This toxicity study is classified as acceptable and satisfies the guideline requirement for early life toxicity study with fish.
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