9,9-bis-(methoxymethyl)fluorene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: GB/T 21809-2008: Chemicals-Test Method of Earthworm Acute Toxicity Test

Principles of method if other than guideline:

- Principle of test: acute toxicity study to earthworms over 14 day period

- Short description of test conditions:
Artificial soil treated with the test substance
Four replicate plus control
Parameters were observed at start of test, 7th day and end of test (day 14)

- Parameters analysed / observed:
Mortality
Abnormal behaviour

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): high-speed blender for 5 minutes
- Controls: artificial soil

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Shanghai Yonghe Biotech Earthworm Breeding Farm
- Age at test initiation: 3 months
- Weight at test initiation (mean and SD): 0.46 +/- 0.08g

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): healthy, no mortality observed

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

20°C +/- 2°C

pH:

6.12 - 6.26

Moisture:

35% (Humidity = 80%)

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Glass 1L, perforated lid
- Amount of soil or substrate: 750g (wet weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 2
- No. of replicates per control: 2

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): Sphagnum Peat 10% (pH 5.5, no visible plant remains), Kaolin Clay 20% (Kaolinite content was 45%), Industrial Sand 70%
- Maximum water holding capacity (in % dry weight): 35%

OTHER TEST CONDITIONS
- Photoperiod: Continuous light
- Light intensity: 600-800 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
2 minutes after start of test, 7 days, 14 days

VEHICLE CONTROL PERFORMED: yes
- Filter paper contact test with chloroacetamide (A.R.) to deterrmine sensitivity of earthworms
- 1 earthworm/concentration
- test duration: 24 hours in dark, 20 +/-2°C
- Observation taken with 30 secs of initiation of test then 24h.

TEST CONCENTRATIONS
- Limit test: 1000 mg/kg
- Range finding study (7 days)
- Test concentrations: 0, 100, 1000 mg/kg
- Results used to determine the conditions for the definitive study: yes

Nominal and measured concentrations:

Nominal: 1000 mg/kg

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Key result

Duration:

14 d

Dose descriptor:

LD50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: No

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 24h LC50 3.63 x 10-3 mg/cm2
- within historical range - 1.5 x 10-3 to 4.5 x 10-3 mg/cm2

Validity criteria fulfilled:

yes

Conclusions:

The 14d LC50 for the test material was was determined to be >1000 mg/kg. No mortality was observed in the test.

Description of key information

In acute toxicity to earthworm study conducted in accordance with GLP, the 14 day LD50 to earthworms was deterrmined to be greater than the highest test concentration (1000mg/kg)

Additional information

It is not appropriate to include an EC50 value for the chemical safety assessment above as this is determined to be greater than the highest concentration tested