Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-766-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From March 29, 2010 to April 15, 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- KL2 as RA study
- Justification for type of information:
- Refer to section 13 for details on the read-across justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: From the series of concentrations the second lowest and the second highest test concentration (125 and 500 µg/L) and the control were analytically verified at the beginning of the test.
- Sampling method: Separate replicates for the test item analysis after 0 and 72 h were prepared without algae. Analysis after 72 h of these concentrations were carried out. Sorption to the walls of the glass container was checked out of one test replicate (prepared with algae) of a concentration level close to the EC50-value (the second highest test concentration of 500 µg/L was analysed).
- Sample storage conditions before analysis: All samples were stored at room temperature until start of analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 10 mg/L was freshly prepared. Dispersion treatment was 5 min ultrasound at room temperature, equilibration phase for 1 h before application was carried out.
- Eluate: NAtural river water
- Differential loading: 62.5 - 125 - 250 - 500 - 1000 µg/L
- Controls: Six replicates (without test item) were tested under the same test conditions as the test replicates. - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata HINDAK
- Strain: SAG 61.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared from Z-Agar. Light intensity amounted 35 - 70 µE x m-2 x s-1 for 24 h per day.
ACCLIMATION
- Culturing media and conditions (same as test or not): Not the same, standard growth medium
- Any deformed or abnormal cells observed: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- After 72 h of exposure, 0.2 – 1 mL aliquots of the test medium (i.e. samples of exposed algal cells) from test concentrations 500 – 1000 µg/L and control were transferred to 10 mL untreated test medium. Algae were then allowed to grow for further 5 days under test conditions. The test item effect was observed to be reversible at these concentrations. Therefore, there is potential for recovery following exposure up to 1000 µg/L (highest test concentration).
For details please refer to the table under information on materials and methods - Hardness:
- Not measured
- Test temperature:
- Mean: 21.5 °C, Min: 21 °C, Max: 22 °C
- pH:
- Nominal test item concentration pH-value
[µg/L] Start; 0 h End; 72 h
1000 8.02 8.01
500 8.03 8.01
250 8.06 8.37
125 8.06 8.92
62.5 8.07 9.07
Control 8.06 9.14 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to information on materials and methods
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): sealed with cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: 9787 cells/mL
- Control end cells density: Mean 2742164 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): None
GROWTH MEDIUM
- Standard medium used: Yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water of the river Leine was used. This river is located near D-31171 Nordstemmen, Marienbergstraße, Germany (52° 10’ 14.98‘’, 9° 46’ 7.64’’). Additionally 50 % of the components concentrations of the dilution water (total application volume 6.5 mL/L) acc. to the guideline were added to enable a sufficient growth of algae
River Leine
Location D-31171 Nordstemmen, Marienbergstrasse
Sampling Date 2009-12-15
Weather on Day of Sampling cloudy, ca. - 1 °C
Colour Yellowish, clear
pH 7.97
Conductivity [µS/cm] 386
DOC [mg C/L] 3.9
TOC [mg C/L] 3.9
Ammonium-N [mg N/L] 0.042
Nitrate-N [mg N/L] 2.62
o-Phosphate-P [mg P/L] 0.062
Total Phosphate [mg P/L] 0.053
Suspended Matter [mg/L] 16.2
Total Hardness [mg CO3/L] 154
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Fluorimeter, via Chlorophyll-a fluorescence (excitation at 436 nm, emission at 685 nm), cell concentrations were related to all cell density values acc. to a calibration curve.
TEST CONCENTRATIONS
- Range finding study: Yes
Results of the Range Finding Test (0 – 72 h)
Nominal
Test Item Concentration
[mg/L] Specific Growth Rate Inhibition [%] Yield Inhibition [%]
10.0 100 100
1.00 89 99
0.10 1 3
0.01 - 6 - 32
0.001 - 7 - 37
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 139.8 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Remarks:
- Corrected for a.i. and 45% sorption
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 269 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (200 - 351)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 200 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 103.95 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Remarks:
- Corrected for a.i. and 45% sorption
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 453 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (388-514)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 235.4 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Remarks:
- corrected for a.i. and 45% sorption
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 277 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 143.9 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Remarks:
- corrected for a.i. and 45% sorption
- Basis for effect:
- other: Yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50:
Rate-related inhibition: 0.74 (CI 0.71 - 0.78) mg/L
Yield Inhibition: 0.36 (CI < 0.35 - 0.36) mg/L - Reported statistics and error estimates:
- EC10-, EC20- and EC50-values of the growth rate and yield
analyses inhibition after 72 h were calculated by sigmoidal dose-response regression and 0 – 100 variable slope equation. Calculation of the confidence intervals of EC10-, EC20- and EC50-values were carried out from the best fit values, the standard error and the t-distribution with the software GraphPad Prism. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the EyC50 and ErC50 (0-72 h) were 277 µg/L(equivalent to 143.9µg a.i./L) and 453µg/L (i.e., equivalent to 235.4µg a.i./L), respectively. The ErC10, EyC10, values were determined at 269 µg/L (i.e., equivalent to 139.8 µg a.i./L), and 200 µg/L (i.e., equivalent to 103.95µg a.i./L) (nominal) respectively
- Executive summary:
A study was conducted to determine the toxicity of the read across substance, N,N,N',N',N''-Pentamethyl-N-C16-18 (even numbered) and C18 unsat.-alkyl-1,3-propanediammonium chloride to aquatic algae, according to OECD Guideline 201, in compliance with GLP.Pseudokirchneriella subcapitatawere exposed for 72 h to nominal concentrations of62.5, 125, 250, 500 and 1000 µg/Lof the test substance.Three replicates were tested for each of the test substance concentrations and six replicates for the control. Analytical dose verification was performed. Chemical analysis of duplicate samples at125 and 500 µg/Ltaken at the beginning (64 - 67 %recovery) and at the end (25 - 31%recovery) of the test indicated biodegradation as possible reason for this decrease is very unlikely considering the short time frame. Also the river water was frozen before use to minimize the microbial activity. The decrease is attributed to additional sorption to suspended matter and DOC due to thermodynamically driven redistribution of the adsorbed fraction. Test substance recovered from the glassware (227 µg/L at concentration level 500 µg/L corresponding to 45 % of the nominal concentration). All effect values are given based on nominal concentrations of the test substance.Growth rate and biomass were recorded throughout the experiment. The EC10 for inhibition of specific growth rate (ErC10) and yield (EyC10) after 72 h were 269 and 200 µg/L, respectively. The EC50 for inhibition of specific growth rate (ErC50) and yield (EyC50) after 72 h were 453 and 277 µg/L, respectively.Under the study conditions, the EyC50 and ErC50 (0 -72 h) were277 µg/L(equivalent to 143.9µg a.i./L) and453µg/L (equivalent to 235.4µg a.i./L), respectively and the ErC10 and EyC10 were269µg/L (equivalent to 139.8µg a.i./L) and 200µg/L(equivalent to 103.95µg a.i./L) (nominal) respectively (Scheerbaum, 2010).
Reference
EC10-, EC20-and EC50- values and 95 % Confidence Intervals of
N,N,N',N',N’’-Pentamethyl-N-tallowalkyl-1,3-propanediammonium chloride (CAS No. 68607-29-4) (0-72 h)
based on nominal test item concentrations [µg/L]
Rate-related inhibition | |
ErC10 | 269 (200 – 351) |
ErC20 | 322 (254 – 388) |
ErC50 | 453 (388 – 514) |
Inhibition of yield | |
EyC10 | 200 (152 – 236) |
EyC20 | 225 (178 – 248) |
EyC50 | 277 (254 – 375) |
Cell Densities
| Nominal | Replicate | Cell density [cells/mL] | ||||||
[µg/L] | No. | 0 h | 24 h | 48 h | 72 h |
| |||
1000 | 1 | 9787 | 23896 | 15906 | 17373 |
| |||
2 | 9787 | 21109 | 19972 | 10638 |
| ||||
3 | 9787 | 19013 | 18628 | 15013 |
| ||||
Mean | 9787 | 21339 | 18169 | 14341 |
| ||||
500 | 1 | 9787 | 26919 | 36676 | 45220 |
| |||
2 | 9787 | 27172 | 32615 | 52557 |
| ||||
3 | 9787 | 44520 | 74567 | 363923 |
| ||||
Mean | 9787 | 32870 | 47953 | 153900 |
| ||||
250 | 1 | 9787 | 35303 | 276296 | 1560834 |
| |||
2 | 9787 | 39524 | 310150 | 1805422 |
| ||||
3 | 9787 | 42678 | 353094 | 2091520 |
| ||||
Mean | 9787 | 39168 | 313180 | 1819259 |
| ||||
125 | 1 | 9787 | 59410 | 586397 | 2864605 |
| |||
2 | 9787 | 68838 | 659571 | 3158989 |
| ||||
3 | 9787 | 73857 | 749999 | 3237056 |
| ||||
Mean | 9787 | 67368 | 665322 | 3086883 |
| ||||
62.5 | 1 | 9787 | 64274 | 745257 | 3107670 |
| |||
2 | 9787 | 69585 | 646980 | 2771469 |
| ||||
3 | 9787 | 78642 | 722533 | 3017782 |
| ||||
Mean | 9787 | 70834 | 704923 | 2965640 |
| ||||
Control | 1 | 9787 | 65675 | 645222 | 2583972 |
| |||
2 | 9787 | 60190 | 554785 | 2438766 |
| ||||
3 | 9787 | 74703 | 806164 | 2544032 |
| ||||
4 | 9787 | 66206 | 809642 | 3065234 |
| ||||
5 | 9787 | 65402 | 695714 | 2922748 |
| ||||
6 | 9787 | 71376 | 715299 | 2898233 |
| ||||
Mean | 9787 | 67259 | 704471 | 2742164 |
|
Evaluation after 72 h
Nominal | Replicate | Growth rate | Rate-related inhibition | Yield | Inhibition of yield | ||
[µg/L] | No. | [d-1] | [%] | [cells/mL] | [%] | ||
1000 | 1 | 0.191 | 89.8 | 7586 | 99.7 | ||
2 | 0.028 | 98.5 | 851 | 100 | |||
3 | 0.143 | 92.4 | 5226 | 99.8 | |||
Mean | 0.121 | 93.6 | 4554 | 99.8 | |||
500 | 1 | 0.510 | 72.8 | 35433 | 98.7 | ||
2 |
| 0.560 | 70.2 | 42770 | 98.4 | ||
3 | 1.21 | 35.8 | 354136 | 87.0 | |||
Mean | 0.76 | 59.6 | 144113 | 94.7 | |||
250 | 1 | 1.69 | 9.94 | 1551047 | 43.2 | ||
2 | 1.74 | 7.36 | 1795635 | 34.3 | |||
3 | 1.79 | 4.75 | 2081733 | 23.8 | |||
Mean | 1.74 | 7.35 | 1809472 | 33.8 | |||
125 | 1 | 1.89 | -0.84 | 2854818 | -4.48 | ||
2 | 1.93 | -2.58 | 3149202 | -15.3 | |||
3 | 1.93 | -3.01 | 3227269 | -18.1 | |||
Mean | 1.92 | -2.14 | 3077096 | -12.6 | |||
62.5 | 1 |
| 1.92 | -2.28 | 3097883 | -13.4 | |
2 | 1.88 | -0.25 | 2761682 | -1.07 | |||
3 | 1.91 | -1.76 | 3007995 | -10.1 | |||
Mean | 1.90 | -1.43 | 2955853 | -8.18 | |||
Control | 1 | 1.86 | 2574185 | ||||
2 | 1.84 | 2428979 | |||||
3 | 1.85 | 2534245 | |||||
4 | 1.92 | 3055447 | |||||
5 | 1.90 | 2912961 | |||||
6 | 1.90 | 2888446 | |||||
Mean | 1.88 | 2732377 |
Section-by-Section and Average Specific Growth Rates of the Control Group
(0 – 72 h)
Replicate No. | Specific growth rate [d-1] | Mean (0 - 72 h) | SD ± | VC % | Mean VC % | |||
section-by-section | ||||||||
0 - 24 h | 24 - 48 h | 48 - 72 h | ||||||
Control | 1 | 1.90 | 2.29 | 1.39 | 1.86 | 0.450 | 24.2 | 26.3 |
2 | 1.82 | 2.22 | 1.48 | 1.84 | 0.371 | 20.2 | ||
3 | 2.03 | 2.38 | 1.15 | 1.85 | 0.634 | 34.2 | ||
4 | 1.91 | 2.50 | 1.33 | 1.92 | 0.586 | 30.6 | ||
5 | 1.90 | 2.36 | 1.44 | 1.90 | 0.465 | 24.5 | ||
6 | 1.99 | 2.31 | 1.40 | 1.90 | 0.459 | 24.2 | ||
Mean | 1.88 |
| ||||||
SD ± | 0.03 |
| ||||||
VC % | 1.63 |
|
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 235.4 µg/L
- EC10 or NOEC for freshwater algae:
- 139.8 µg/L
Additional information
A study was conducted to determine the toxicity of the read across substance, N,N,N',N',N''-Pentamethyl-N-C16-18 (even numbered) and C18 unsat.-alkyl-1,3-propanediammonium chloride to aquatic algae, according to OECD Guideline 201, in compliance with GLP. Pseudokirchneriella subcapitata were exposed for 72 h to nominal concentrations of 62.5, 125, 250, 500 and 1000 µg/L of the test substance. Three replicates were tested for each of the test substance concentrations and six replicates for the control. Analytical dose verification was performed. Chemical analysis of duplicate samples at 125 and 500 µg/L taken at the beginning (64 - 67 % recovery) and at the end (25 - 31% recovery) of the test indicated biodegradation as possible reason for this decrease is very unlikely considering the short time frame. Also the river water was frozen before use to minimize the microbial activity. The decrease is attributed to additional sorption to suspended matter and DOC due to thermodynamically driven redistribution of the adsorbed fraction. Test substance recovered from the glassware (227 µg/L at concentration level 500 µg/L corresponding to 45 % of the nominal concentration). All effect values are given based on nominal concentrations of the test substance. Growth rate and biomass were recorded throughout the experiment. The EC10 for inhibition of specific growth rate (ErC10) and yield (EyC10) after 72 h were 269 and 200 µg/L, respectively. The EC50 for inhibition of specific growth rate (ErC50) and yield (EyC50) after 72 h were 453 and 277 µg/L, respectively. Under the study conditions, the EyC50 and ErC50 (0 -72 h) were 277 µg/L (equivalent to 143.9µg a.i./L) and 453 µg/L (equivalent to 235.4 µg a.i./L), respectively and the ErC10 and EyC10 were 269 µg/L (equivalent to 139.8 µg a.i./L) and 200 µg/L(equivalent to 103.95 µg a.i./L) (nominal) respectively (Scheerbaum, 2010).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1