Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 284-854-1 | CAS number: 84988-66-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Lawsonia inermis, Lythraceae.
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- other: Mutation Research, 2003
- Adequacy of study:
- key study
- Study period:
- na
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- 1997
- GLP compliance:
- yes
- Type of assay:
- mammalian bone marrow chromosome aberration test
Test material
- Reference substance name:
- Lawsonia inermis, ext.
- EC Number:
- 284-854-1
- EC Name:
- Lawsonia inermis, ext.
- Cas Number:
- 84988-66-9
- Molecular formula:
- na
- IUPAC Name:
- Lawsonia inermis, ext.
- Reference substance name:
- 2-hydroxy-1,4-naphthoquinone
- Cas Number:
- 201-496-3
- Molecular formula:
- C10H6O3
- IUPAC Name:
- 2-hydroxy-1,4-naphthoquinone
- Test material form:
- solid: particulate/powder
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- not specified
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- Methylcellulose. Positive control; Vehicle was water
- Details on exposure:
- 300mg/kg
- Duration of treatment / exposure:
- not specified
- Frequency of treatment:
- once
Doses / concentrations
- Dose / conc.:
- 300 mg/kg diet
- No. of animals per sex per dose:
- not specified
- Control animals:
- yes
- Positive control(s):
- cyclophosphamide (CPA) 50 mg/kg
Results and discussion
Test results
- Key result
- Sex:
- not specified
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- not specified
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The results of a further mouse bone marrow MN test with Henna containing HNQ did not indicate an increase in MN frequency at any dose, even though bone marrow toxicity was induced.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
