Reaction mass of dipotassium 3,3'-sulphonylbis(benzenesulphonate) and potassium 3-(phenylsulphonyl)benzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 Feb 2018 - 17 April 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Reference Identification: KSS-FR; Reaction mass of dipotassium 3,3’-sulphonylbis(benzenesulphonate) and potassium 3- (phenylsulphonyl)benzenesulphonate
CAS# 63316-33-6 and CAS# 63316-43-8
EC# 915-932-1
Label Identification: Arichem, LLC; 187 Sloss Industries Road; Ariton, AL 36311 USA
Product Sample: Potassium Diphenylsulfone Sulfonate; (KSS-FR) Lot: 1801-08
Date & Quantity Received: 29 Jan 18; 108.4 g (GW)
Physical Description: White powder
Storage Conditions: Room temperature
Purity: 92.6% active ingredient

Analytical monitoring:

no

Remarks:

Substance is a non-volatile solid and stable in water at environmental pH

Vehicle:

no

Details on test solutions:

0.108 g of test item was disolved in 1000 mL of dilution water and mixed for 15 minutes

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna
Justification of Species: One of the daphnid species specified in the regulations
Source: STILLMEADOW, Inc. culture laboratory
Age: No greater than 24 hours old at dosing
- Range-finding DOB: 20 Feb 18
- Limit Test DOB: 27 Feb 18
Quantity Range-finding: 10 per test concentration and control
Quantity Limit Test: 20 per test concentration and control

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

Range-finding: 215 mg/L CaCO3
Limit Test: 180 mg/L CaCO3

Test temperature:

Target: 20 +/- 1 deg. Celsius
Actual: 21°C

pH:

Range finding: 7.3
Limit Test: 7.6

Dissolved oxygen:

Target: 60 - 105% saturation at dosing
Actual Range-finding: 91-95%
Actual Limit Test: 90-94%

Conductivity:

673 - 685 µmhos/cm

Nominal and measured concentrations:

Range Finding: 0.01 mg/L, 0.1 mg/L, 1.0 mg/L, 10 mg/L, and 100 mg/L
Limit Test: 100 mg/L

Details on test conditions:

Test Chambers: 250 mL glass beakers; filled with 200 mL of the appropriate solution
16-hours light / 8-hours dark cycle

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Temperature, pH, Dissolved Oxygen, and Conductivity were all maintained within the desired test parameters throughout the study.

Limit Test Survival:

Conc. (mg/L)	Replicate	Number of Surviving Organisms			Survival
		0 Hours	24 Hours	48 Hours
Control	A	5	5	5	100%
	B	5	5	5
	C	5	5	5
	D	5	5	5
100	A	5	5	4*	80%
	B	5	5	4*
	C	5	5	4*
	D	5	5	4*

Note:All organisms had no observable abnormalities unless otherwise indicated.

*Solution was clear. Organisms observed moving very slowly.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was determined to be >100 mg/L

Executive summary:

In a GLP guidline study conducted according to OECD 202, the substance was evaluated for toxicity to Daphnia Magna in a 48-hour static test at a limit dose of 100 mg/L. Four of the five test organisms (80%) survived the 48 -hour test period, with all organisms (100%) surviving the first 24 hours. Given these results, the EC50 was determined to be >100 mg/L.

Description of key information

In a GLP guidline study conducted according to OECD 202, the substance was evaluated for toxicity to Daphnia Magna in a 48-hour static acute toxicity test at a limit dose of 100 mg/L. Four of the five test organisms (80%) survived the 48 -hour test period, with all organisms (100%) surviving the first 24 hours. Given these results, the EC50 was determined to be >100 mg/L.

Additional information

EC50 >100 mg/L