Oxirane, 2,2'-[methylenebis[(2,6-dimethyl-4,1-phenylene)oxymethylene]]bis-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 July 1995 to 19 July 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Japanese Industrial Standard (JIS K 0102-1993-71.) "Testing methods for industrial waste water".

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Remarks:

HCO-20 and HCO-40

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- HCO-20 and HCO-40 were used as dispersants
- Method: The test material and HC0-20 (20 times amount of the test material) and HC0-40 (20 times amount of the test material) were dissolved in acetone. After acetone was evaporated from it, ion-exchanged water was added to obtain 1000 mg/L of stock solution.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange-red killifish
- At reception, fish showing abnormal signs were removed. Then fish were reared according to flow through system for 6 days after an external disinfection.
- Length at study initiation: average 2.6 cm
- Weight at study initiation: average 0.16 g

ACCLIMATION
-Ffish were acclimatised after external disinfections. During the period, abnormal fish were removed. Fish were reared according to flow through system at a temperature of 25 ± 2°C for 19 days. After removing the abnormal fish and performing the external disinfection again, fish were reared according to flow through system for 27 days.
- Fish which had passed the HgCl2-test (Kenji Tabata (1972) Journal of Water and Waste (14) 1297-1303) were subjected to the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

25 ± 2°C

pH:

Initiation: 7.8 - 7.9
Termination: 7.8 - 8.0

Dissolved oxygen:

Initiation: 7.6 mg/L
Termination: 6.6 - 6.9 mg/L

Nominal and measured concentrations:

Nominal: 0.479, 0.638, 0.848, 1.13 and 1.50 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Round glass vessel
- Fill volume: 4L/level
- Renewal rate of test solution (frequency/flow rate): Renewal of test water was performed every 8 - 16 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Underground water pumped up from the ground of the testing laboratory site was used. The water was analysed and measured every 6 months in the laboratory.
- The quality of dilution water was confirmed to meet the ministerial ordinance of the Ministry of Public Welfare (December 21, 1992), the water quality criteria for fisheries (Shadanhozin Nihon Suisansigen Hogokyokai, March 1983), and OECD Guideline for Testing of Chemicals 210, Fish, Early-life Stage Toxicity Test in measured and analysed items.

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The 48-hr LC50 value was estimated by Doudoroff method.

Sublethal observations / clinical signs:

Table 1: Cumulative Mortality

Concentration (mg/L)	Cumulative Mortality (%)
	2 Hours	24 Hours	48 Hours
Control	-	0	0
0.479	-	0	0
0.638	-	0	0
0.848	-	0	30
1.13	-	20	40
1.50	-	60	100

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of this study, the 48 hour LC50 value to Oryzias Iatipes was determined to be 1.18 mg/L.

Executive summary:

The acute toxicity of the test material to fish was investigated in accordance with Japanese Industrial Standard (JIS K 0102-1993-71.) titled "Testing methods for industrial waste water". The testing was performed under GLP conditions.

Oryzias Iatipes (Orange-red killifish) were exposed to the test material for 48 hours in a semi-static test system. Ten fish were treated at each of the following concentrations: 0.479, 0.638, 0.848, 1.13 and 1.50 mg/L.

The 48-hr LC50 value was estimated by Doudoroff method.

Under the conditions of this study, the 48 hour LC50 value was 1.18 mg/L.

Description of key information

Under the conditions of this study, the 48 hour LC50 value to Oryzias Iatipes was determined to be 1.18 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.18 mg/L

Additional information

The acute toxicity of the test material to fish was investigated in accordance with Japanese Industrial Standard (JIS K.0102-1993-71.) titled "Testing methods for industrial waste water". The testing was performed under GLP conditions. The study was awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997).

Oryzias Iatipes (Orange-red killifish) were exposed to the test material for 48 hours in a semi-static test system. Ten fish were treated at each of the following concentrations: 0.479, 0.638, 0.848, 1.13 and 1.50 mg/L.

The 48-hr LC50 value was estimated by Doudoroff method.

Under the conditions of this study, the 48 hour LC50 value was 1.18 mg/L.