Registration Dossier
Registration Dossier
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EC number: 916-604-0 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
In the acute immobilization test with Daphnia magna the effects of the test item BasicBrown17 were determined according to OECD guideline 202.
The study was conducted under semi-static conditions over a period of 48 hours with 5 concentrations of the test item BasicBrown17 in the range of 4.27 to 100 mg/L, prepared in a geometric series with a separation factor of 2.2.All tested concentration levels were visually clear throughout the exposure and exhibited a concentration-related red-orange coloring. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control.The concentrations of the test item BasicBrown17 were analytically verified via HPLC-DAD in fresh media of all concentration levels and the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 95 to 109% of the nominal values and 88 to 109% in old media at renewal and at the end of the test (24 and 48 hours). Since the measured test item concentrations were within the range of ±20% of the nominal concentrations, the effect concentrations are based on the nominal concentrations of the test item BasicBrown 17.The validity criteria of the test guidelines were fulfilled.Based on the nominal concentrations of the test item Basic Brown 17, the 48 hour EC50 for Daphnia magna was 21.0 mg/L (95% confidence limits : 10.3-42.8 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 21 mg/L
Additional information
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