2-methoxy-2-methylheptane

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

8 February 2017 - 8 March 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Method for Testing the Biodegradability of Chemical Substances by Microorganisms

Version / remarks:

Method stipuated in the "Testing Methods for New Chemical Substances" (March 31, 2011, No. 0331-7, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labur and Welfare; March 29, 2011, No. 5, Manufacturing Industries Bureau, Ministry of Economy, Trade and Industry; No. 110331009, Environmental Policy Bureau, Ministry of the Environment, Japan; latest revision, December 21, 2015, No. 1221-1, Pharmaceutical and Food Safety Bureau, Ministry of Economy, Trade and Industry; No. 1512211, Environmental Policy Bureau, Ministry of the Environment, Japan

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-methoxy-2-methylheptane

EC Number:

816-311-7

Cas Number:

76589-16-7

Molecular formula:

C9H20O

IUPAC Name:

2-methoxy-2-methylheptane

Test material form:

liquid

Specific details on test material used for the study:

Chemical name: 2-Methoxy-2-methylheptane
CAS number: 76589-16-7 (FLAT)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

On-site sludge sampling was carried out at 10 locations in Japan (samples were from surface water and surface soil of rivers, lakes and inland sea, and return sludge from sewage pants). Activated sludge, which was prepared and was controlled in this laboratory according to the test method described above, was used in this study. The activated sludge, which was cultivated for 19.5 hours after feeding with the sythetic sewage, was used. The synthetic sewage was prepared according to the following method; glucose, peptone, and potassium dihydrogenphosphate were dissolved in purified water, nd the pH of the solution was adjusted to 7±1.0.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Concentration of test item: 100mg/L
Concentration of ativated sludge: 30mg/L (as concentration of suspended solid)
Volume of test solution: 300mL
Incubation temperature: 25±1°C

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

The average percentage biodegradation was regarded as 0% because the calculated average value was negative.
See attachment.

BOD5 / COD results

BOD5 / COD

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Remarks on result:

other: BOD

Remarks:

7th day

Results with reference substance:

BOD sludge + aniline: 76.6mg on the 28th day.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item did not undergo biodegradation under the test conditions of this study.