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EC number: 257-861-2 | CAS number: 52338-87-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The test item was a mixture of the reaction products (1-(3-(Dimethylamino)propyl)urea (CAS 31506-43-1, EC 401-950-2, mono-DMAPAU) and 1,3-bis[3-(dimethylamino)propyl]urea (CAS 52338-87-1, EC 257-861-2, bis-DMAPAU)
Both source (1-(3-(Dimethylamino)propyl)urea (CAS 31506-43-1, EC 401-950-2) )and target 1,3-bis[3-(dimethylamino)propyl]urea (CAS 52338-87-1, EC 257-861-2) substances are based on the reaction product of dimethylaminopropylamine (DMAPA) and urea.
The main product of this reaction is the singly-substituted urea product, (3-(dimethylamino)propyl) urea (or mono-DMAPAU)
However, an unavoidable side-reaction is the formation of the doubly-substituted product, 1,3-bis[3-(dimethylamino)propyl]urea, which is formed by the disproportionation of mono-DMAPAU.
The test item contains approximately 15% of 1,3-bis[3-(dimethylamino)propyl]urea.
Read-across is also claimed based on structural similarity and properties of both reaction products. Justification attached. - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- not specified
- Principles of method if other than guideline:
- In accordance with Japanese Industrial Standards (JIS) K 0102- 1998section 14.1
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name: Reaction product of N,N-dimethylpropane-1,3-diyldiamine and Urea, main product:1-[3-(dirnethylamino)propyl]urea
Appearance: Clear or cloudy liquid
Boiling point: >I50 "C
Solubility Water: 100%
Stability: The test item was stable under the storage conditions, as shown by the finding that IR spectra of the test item before the experimental start and after the experimental completion were identica - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- DOC removal
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 1
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Parameter:
- % degradation (TOC removal)
- Value:
- 2
- Sampling time:
- 28 d
- Details on results:
- Results:
1) Percentagebiodegradation by BOD 0%, 3%, 1%, average 1%
2) Percentage biodegradationby TOC 2%, 2%, 3% average 2%
3) Percentage biodegradation by HPLC 1%, 1%, 0% average 1% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- A mixture of the reaction products (1-(3-(Dimethylamino)propyl)urea (CAS 31506-43-1, EC 401-950-2, monoDMAPAU) )and 1,3-bis[3-(dimethylamino)propyl]urea (CAS 52338-87-1, EC 257-861-2, bisDMAPAU) was tested in an OECD 301c modified MITI test for biodegradation. Under the present test conditions, the test item was not biodegraded.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- not specified
- Principles of method if other than guideline:
- In accordance with Japanese Industrial Standards (JIS) K 0102- 1998section 14.1
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name: Reaction product of N,N-dimethylpropane-1,3-diyldiamine and Urea, main product:1-[3-(dirnethylamino)propyl]urea
Appearance: Clear or cloudy liquid
Boiling point: >I50 "C
Solubility Water: 100%
Stability: The test item was stable under the storage conditions, as shown by the finding that IR spectra of the test item before the experimental start and after the experimental completion were identica - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- DOC removal
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 1
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Parameter:
- % degradation (TOC removal)
- Value:
- 2
- Sampling time:
- 28 d
- Details on results:
- Results:
1) Percentagebiodegradation by BOD 0%, 3%, 1%, average 1%
2) Percentage biodegradationby TOC 2%, 2%, 3% average 2%
3) Percentage biodegradation by HPLC 1%, 1%, 0% average 1% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- A mixture of the reaction products (1-(3-(Dimethylamino)propyl)urea (CAS 31506-43-1, EC 401-950-2, monoDMAPAU) )and 1,3-bis[3-(dimethylamino)propyl]urea (CAS 52338-87-1, EC 257-861-2, bisDMAPAU) was tested in an OECD 301c modified MITI test for biodegradation. Under the present test conditions, the test item was not biodegraded.
Referenceopen allclose all
Conditions
of cultivation
1)
Concentration of test item 100mglL
2)
Concentration of activated sludge 30 mg/L (as
the concentration of suspended solid)
3)
Volume of test solution 300 mL
4)
Cultivation temperature 25+/-1 degrees C
5)
Cultivation duration 28 days
Measurement
and analysis
1)
Measurement of biochemical oxygen demand (BOD) by means of a closed
system oxygen consumption measuring
apparatus
2)
Determinationof dissolved organic carbon by means of a total organic
carbon analysis (TOC)
3)
Determination of test substance (DMAPAU)by means of high-performance
liquid chromatography (HPLC)
4)
Determination of urea by means of hgh-performance liquid chromatography
(HPLC)
Results:
1)
Percentagebiodegradation by BOD 0%, 3%, 1%,average1%
2)
Percentage biodegradationby TOC 2%, 2%, 3% average 2%
3)
Percentage biodegradation by HPLC1%,1%,0%
average 1%
Conditions
of cultivation
1)
Concentration of test item 100mglL
2)
Concentration of activated sludge 30 mg/L (as
the concentration of suspended solid)
3)
Volume of test solution 300 mL
4)
Cultivation temperature 25+/-1 degrees C
5)
Cultivation duration 28 days
Measurement
and analysis
1)
Measurement of biochemical oxygen demand (BOD) by means of a closed
system oxygen consumption measuring
apparatus
2)
Determinationof dissolved organic carbon by means of a total organic
carbon analysis (TOC)
3)
Determination of test substance (DMAPAU)by means of high-performance
liquid chromatography (HPLC)
4)
Determination of urea by means of hgh-performance liquid chromatography
(HPLC)
Results:
1)
Percentagebiodegradation by BOD 0%, 3%, 1%,average1%
2)
Percentage biodegradationby TOC 2%, 2%, 3% average 2%
3)
Percentage biodegradation by HPLC1%,1%,0%
average 1%
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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