4,4'-(2,3-epoxypropoxy)-2,2'- dimethyl-5,5'-tert-butyldiphenylsulphide

Endpoint summary

Description of key information

Skin Irritation

Under the conditions of the study, the test material is not irritating to the skin.

Eye Irritation

Under the conditions of the study, the test material was not irritating to eyes.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5 January 2011 to 7 January 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)

Version / remarks:

2010

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EpiSkin™ SOP, ECVAM Skin Irritation Validation Study: Validation of the EpiSkin™ test method 15 min - 42 hours for the prediction of acute skin irritation of chemicals.

Version / remarks:

Version 1.8 (February 2009)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test system:

human skin model

Source species:

human

Cell type:

non-transformed keratinocytes

Justification for test system used:

Recommended in international guidelines

Vehicle:

unchanged (no vehicle)

Details on test system:

RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE
- Model used: EpiSkin™ Small Model (EpiSkin™SM)
- Source: Skinethic, Nice, France
- Tissue batch number(s): 11-EKIN-001
- Expiry date: 10 January 2011
- EpiSkin™ Small Model (EpiSkin™SM), is a three-dimensional human epidermis model. Adult human-derived epidermal keratinocytes are seeded on a dermal substitute consisting of a collagen type I matrix coated with type IV collagen. A highly differentiated and stratified epidermis model is obtained after 13-day culture period comprising the main basal, supra basal, spinous and granular layers and a functional stratum corneum. Its use for skin irritation testing involves topical application of test materials to the surface of the epidermis, and the subsequent assessment of their effects on cell viability.

KIT RECEPTION
- The colour of the agar medium used for transport was checked for its pH: orange colour = good or yellow or violet colour = not acceptable.
- The colour of the temperature indicator was inspected to verify that the kit has not been exposed to a temperature above 40°C: the indicator changes from white to grey at 40°C.
- The kit was found to be in good order at reception.

INDICATOR FOR POTENTIAL FALSE VIABILITY
- Check-method for possible direct MTT reduction with test material: Approximately 10 mg of test material was added to 2 mL MTT ready to use solution and mixed. The mixture was incubated for three hours at room temperature protected from light and then any colour change was observed: Test materials which do not interact with MTT turn yellow and test materials interacting with MTT turn blue or purple. If the MTT solution colour becomes blue or purple, the test material interacts with the MTT. It is then necessary to evaluate the part of optical density (OD) due to the non-specific reduction of the MTT (i.e. by using killed epidermis).
- Additional controls for MTT direct interacting chemicals: For an MTT-interacting test mateiral previously detected, and in addition to the normal procedure, 3 killed treated tissues and 3 killed negative control tissues are used for the MTT evaluation in one run (untreated killed tissues may exhibit little residual NADH and dehydrogenase associated activity). The batch of killed tissues can be different than the batches of the living tissues. The same treatment steps are followed at these tissues as the living tissues.
- Killed epidermis for MTT-interaction substances: Living epidermis are placed at -20°C (or -80°C) for at least 48 hours. Before use, the killed tissues are de-frozen at room temperature (app. 1 hour in 2.2 mL of assay medium). Further use of killed tissues is similar to living tissues.
- Check-method to detect the colouring potential of test materials: Prior to treatment, chemicals were evaluated for their intrinsic colour or ability to become coloured in contact with water (simulating a tissue humid environment). An amount of 10 mg of the test material was added to 90 μL of water. The mixture was shaken for about 15 minutes and then colour checked (unaided visual assessment). If a coloured solution is detected or the test material has an intrinsic colour, the Non Specific Colour % (NSC %) is determined in order to evaluate the ability of test material to stain the epidermis by using additional control tissue(s).
- Additional control(s) for dyes and chemicals able to colour the tissue: For dyes or chemicals able to colour the tissue previously detected, in addition to the normal procedure, at least 1 additional chemical-treated tissue is used for the nonspecific OD evaluation. This tissue follows the same treatment steps than other tissues except for the MTT step: MTT incubation is replaced by incubation with fresh assay medium. OD readings are made following the same conditions as for other tissues.

PERFORMANCE OF THE STUDY
PRE-INCUBATION (DAY [-1])
- The “maintenance medium” was pre-warmed to 37°C. The appropriate number of an assay plate wells were filled with the pre-warmed medium (2 mL per well). The epidermis units were placed with the media below them, in contact with the epidermis into each prepared well and then incubated overnight at 37°C in an incubator with 5% CO2.

APPLICATION AND RINSING (DAY 0)
- 10 mg of test material was applied evenly to the epidermal surface of each of the three test skin units, then 30 μL distilled water was added to the test material to ensure good contact with the epidermis. 10 μL PBS was added to each of the three negative control skin units and 10 μL SDS was added to each of the three positive control skin units
- The plates with the treated epidermis units were incubated for the exposure time of 15 minutes (± 0.5 minutes) at room temperature (18 - 28°C).

TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment / exposure: room temperature
- Temperature of post-treatment incubation: 37°C

REMOVAL OF TEST MATERIAL AND CONTROLS
- After the incubation time the EPISKIN-SM units were removed and rinsed thoroughly with PBS 1x solution (0.9%) to remove all of the test material from the epidermal surface. The rest of the PBS was removed from the epidermal surface with a suitable pipette tip linked to a vacuum source care was taken to avoid the damage of epidermis.
- After rinsing the units were placed into the plate wells with fresh pre-warmed “maintenance medium” (2 mL/well) below them and then incubated for 42 hours (± 1 hour) at 37°C in an incubator with 5% CO2.

MTT TEST AFTER 42 HOURS INCUBATION (DAY 2)
- After the 42 hours incubation the EPISKIN-SM units were transferred into the MTT solution filled wells (2 mL of 0.3 mg/mL MTT per well) and then incubated for 3 hours (± 5 min) at 37°C in an incubator with 5% CO2 protected from light.

FORMAZAN EXTRACTION (DAY 2)
- At the end of incubation with MTT a formazan extraction was undertaken: A disk of epidermis was cut from the unit (this involves the maximum area of the disk) using a biopsy punch (supplied as part of the kit). The epidermis was separated with the aid of forceps and both parts (epidermis and collagen matrix) were placed into a tube of 500 μL acidified isopropanol (one tube corresponding to one well of the tissue culture plate).
- The capped tubes were thoroughly mixed by using a vortex mixer to achieve a good contact of all of the material with the acidified isopropanol then incubated for about two hours at room temperature protected from light with gentle agitation (~150 rpm) for formazan extraction.

NUMBER OF REPLICATE TISSUES: 3

CELL VIABILITY MEASUREMENTS (DAY 2)
- Following the formazan extraction, 2×200 μL sample from each tube was placed into the wells of a 96-well plate (labelled appropriately) and read the OD (Absorbance / Optical Density) of the samples in a 96-well plate spectrophotometer at 540 nm using acidified isopropanol solution as the blank (6×200 μL).


CALCULATIONS OF VIABILITY PERCENTAGES

Data calculation for normal test materials
Blank:
– The mean of the 6 blank OD values is calculated
Negative control:
– Individual negative control OD values are corrected with the mean blank OD:
OD Negative Control (ODNC) = ODNCraw – ODblank mean
– The corrected mean OD of the 3 negative control values are calculated: this corresponds to 100 % viability
Positive control:
– Individual positive control OD values are corrected with the mean blank OD:
OD Positive Control (ODPC) = ODPCraw – ODblank mean
– The corrected mean OD of the 3 positive control values are calculated
– The % viability for each positive control replicate is calculated relative to the mean negative control:
% Positive Control 1 = (ODPC1 / mean ODNC) ×100
% Positive Control 2 = (ODPC2 / mean ODNC) ×100
% Positive Control 3 = (ODPC3 / mean ODNC) ×100
– The mean value of the 3 individual viability % for positive control is calculated:
Mean PC % = (%PC1 + %PC2 + %PC3) / 3
Test material:
– Individual test material OD values are corrected with the mean blank OD:
OD Treated Tissue (ODTT) = ODTTraw – ODblank mean
– The corrected mean OD of the 3 test material values are calculated
– The % viability for each test material replicate is calculated relative to the mean negative control:
% Treated Tissue 1 = (ODTT1 / mean ODNC) ×100
% Treated Tissue 2 = (ODTT2 / mean ODNC) ×100
% Treated Tissue 3 = (ODTT3 / mean ODNC) ×100
– The mean value of the 3 individual viability % for test material is calculated
Mean TT % = (%TT1 + %TT2 + %TT3) / 3


INTERPRETATION OF TEST RESULTS
- A test material is considered to be irritant to skin (R38), if the mean relative viability after 15 minutes exposure and 42 hours post incubation is less or equal (≤) to 50% of the negative control.
- Mean tissue viability % is ≤ 50 %: Irritant (I) R38
- Mean tissue viability % is > 50 %: Non Irritant (NI)

Control samples:

yes, concurrent negative control

yes, concurrent positive control

Amount/concentration applied:

TEST MATERIAL
- Amount(s) applied: 10 mg with 30 μL distilled water to ensure good contact with the epidermis

NEGATIVE CONTROL
- Amount(s) applied: 10 µL

POSITIVE CONTROL
- Amount(s) applied: 10 µL
- Concentration: 5%

Duration of treatment / exposure:

15 minutes (± 0.5 minutes)

Duration of post-treatment incubation (if applicable):

42 hours (± 1 hour)

Number of replicates:

3 replicates per test material, 3 negative controls and 3 positive controls were used

Irritation / corrosion parameter:

% tissue viability

Run / experiment:

Mean

Value:

93

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

valid

Other effects / acceptance of results:

VALIDITY OF THE TEST
- The mean OD value of the three negative control tissues was 0.795. The positive control result showed 25% viability. Each standard deviation value (SD) of the % viability was below 18. All validity criteria were within acceptable limits and therefore the study can be considered as valid.

INDICATOR FOR POTENTIAL FALSE VIABILITY
- Possible direct MTT reduction with test material: No colour change was observed after three hours of incubation. The test material did not interact with the MTT, therefore additional controls and data calculations were not necessary. A false estimation of viability can be precluded.
- Colouring potential of test material: The test material showed no ability to become coloured in contact with water, therefore additional controls and data calculations were not necessary. A false estimation of viability can be precluded.

CELL VIABILITY
- The test material treated tissue viability was 93% after treatment.

Table 1: Optical density (OD) measured at 540 nm of each replicate and calculated % viability of the cells

Treatment		Optical Density (OD)	Viability (%)
Negative Control	1	0.859	108
	2	0.661	83
	3	0.865	109
	Mean	0.795	100
	Standard deviation		14.59
Positive Control	1	0.176	22
	2	0.197	25
	3	0.223	28
	Mean	0.198	25
	Standard deviation		11.86
Test Material	1	0.856	108
	2	0.713	90
	3	0.648	82
	Mean	0.739	93
	Standard deviation		14.37

Interpretation of results:

other: Not classified in accordance with EU Criteria

Conclusions:

Under the conditions of this study, the test material is not irritating to the skin.

Executive summary:

The skin irritation potential of the test material was investigated in accordance with the standardised guideline OECD 439, under GLP conditions.

Disks of EPISKIN (three units / treatment) were treated with the test material and incubated for 15 minutes at room temperature. Exposure of test material was terminated by rinsing with PBS 1x solution (0.9%). Epidermis units were then incubated at 37°C for 42 hours. The viability of each disk was assessed by incubating the tissues for 3 hours with MTT solution. The formazan precipitated was then extracted using acidified isopropanol and quantified spectrophotometrically.

SDS 5% and PBS treated epidermis were used as positive and negative controls respectively. For each treated tissue viability was expressed as a % relative to negative control. The test material is considered to be irritant to skin, if the mean relative viability after 15 minutes exposure and 42 hours post incubation is less or equal (≤) to 50% of the negative control.

All validity criteria were within acceptable limits and therefore the study can be considered as valid. The test material treated tissues had a mean tissue viability of 93% after treatment.

Under the conditions of this study, the test material is not irritating to the skin.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2 December 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 438 (Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants)

Version / remarks:

2009

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Species:

chicken

Strain:

other: ROSS 308

Details on test animals or tissues and environmental conditions:

SOURCE OF COLLECTED EYES
- Chicken heads were collected after slaughter in a commercial abattoir from chickens which are used for human consumption. Heads were collected by a slaughter house technician and heads transported to the testing laboratory at ambient temperature at the earliest convenience.
- After collection, the heads were inspected for appropriate quality and wrapped with tissue paper moistened with saline, then placed in a plastic box which was closed (4-5 heads per box). The heads were received at the testing laboratory and processed within approximately 2 hours of collection.

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent positive control

yes, concurrent negative control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: 30 µg

Duration of treatment / exposure:

10 seconds

Number of animals or in vitro replicates:

3 replicates for the test material and positive control treated eyes and a single negative control treated eye.

Details on study design:

SELECTION AND PREPARATION OF ISOLATED EYES
- Eye selection: After removing the head from the plastic box, it was put on soft paper. The eyelids were carefully cut away with scissors, avoiding damaging the cornea. One small drop of fluorescein solution 2 (w/v) % was applied onto the cornea surface for a few seconds and subsequently rinsed off with 20 mL isotonic saline. Then the fluorescein-treated cornea was examined with hand-held slit lamp or slit lamp microscope, with the eye in the head, to ensure that the cornea was not damaged. If the cornea was in good condition, the eyeball was carefully removed from the orbit.
- Preparation of eyes: The eye ball was carefully removed from the orbit by holding the nictitating membrane with a surgical forceps, while cutting the eye muscles with bent scissors. Care was taken to remove the eyeball from the orbit without cutting off the optical nerve too short. The procedure avoided pressure on the eye while removing the eyeball from the orbit, in order to prevent distortion of the cornea and subsequent corneal opacity. Once removed from the orbit, the eye was placed onto damp paper and the nictitating membrane was cut away with other connective tissue. The prepared eyes were kept on the wet papers in a closed box so that the appropriate humidity was maintained.

EQUILIBRATION AND BASELINE RECORDINGS
- The prepared eye was placed in a steel clamp with the cornea positioned vertically with the eye in the correct relative position (same position as in the chicken head). Again avoiding too much pressure on the eye by the clamp. Because of the relatively firm sclera of the chicken eyeball, only slight pressure was needed to fix the eye properly. The clamp with the eyeball was transferred to a chamber of the superfusion apparatus. The clamp holding the eye was positioned in such a way that the entire cornea was supplied with isotonic saline dripping from a stainless steel tube, at a rate of approximately 2 or 4 drops/minute. The door of the chamber was closed except for manipulations and examinations, to maintain temperature and humidity.
- The appropriate number of eyes was selected and after being placed in the superfusion apparatus, were examined again with the slit lamp microscope to ensure that they were in good condition. The focus was adjusted to see clearly the isotonic saline which was flowing on the cornea surface. Eyes with a high baseline fluorescein staining (i.e., > 0.5) or corneal opacity score (i.e., > 0.5) were rejected. The cornea thickness was measured, any eye with cornea thickness deviating more than 10% from the mean value for the eyes, or eyes that showed any other signs of damage, were rejected and replaced. If the selected eyes were appropriate for the test, acclimatisation started and was conducted for approximately 45 to 60 minutes. Temperature of the circulating water was verified to ensure that all chambers were in the range of 32 ± 1.5°C during the acclimatisation and treatment periods.
- The base line assessments: At the end of the acclimatisation period, a zero reference measurement was recorded for cornea thickness and opacity to serve as a base line (t = 0) for each individual eye. The cornea thickness of the eyes should not increase by more than 5 - 7% between the -45 and the zero time. Slight changes in thickness (+2 to -3%) were observed in the eyes, this is considered normal when maintaining enucleated eyes. Following the equilibration period, the fluorescein retention was measured. Base line values were required to evaluate any potential test material related effects after treatment; the location of any minor findings were marked on the record sheet as a drawing. All eyes were considered to be suitable for the assay.

NUMBER OF REPLICATES: 3 replicates for the test material and positive control treated eyes and a single negative control treated eye.

NEGATIVE CONTROL USED
- Sodium chloride (Salsol solution 0.9%)

POSITIVE CONTROL USED
- Imidazole

APPLICATION DOSE AND EXPOSURE TIME
- 30 µg of test material was uniformly applied by powdering the entire surface of the cornea, taking care not to damage or touch the cornea with the application equipment. The positive control eyes were treated in a similar way with 30 μg of imidazole. The negative control eye was treated with 30 μL of isotonic saline.

REMOVAL OF TEST MATERIAL
- The time of application was observed, then after an exposure period of 10 seconds the cornea surface was rinsed thoroughly with 20 mL isotonic saline at ambient temperature, taking care not to damage the cornea but attempting to remove all the residual test material if possible.

OBSERVATION PERIOD
- The control eye and test eyes were evaluated pre-treatment and at approximately 30, 60, 75, 120, 180 and 240 minutes after the post-treatment rinse. Minor variations within ± 5 minutes were considered acceptable.
- The cornea thickness and cornea opacity were measured at all time points. Fluorescein retention was measured on two occasions, at base line (t = 0) and 30 minutes after the post-treatment rinse.

TREATMENT OF DATA
- Cornea swelling was calculated according to the following formulae:
CS at time t = (CT at time t –CT at t=0 / CT at t=0) x 100
Mean CSmax at up to 75 min = [FECSmax(30min to 75min)+ SECSmax(30min to 75min) + TECSmax(30min to 75min)] / 3
Mean CSmax at up to 240 min = [FECSmax(30min to 240min)+ SECSmax(30min to 240min) + TECSmax(30min to 240min)] / 3
CS = cornea swelling
CT = cornea thickness
FECS = first eye cornea swelling
SECS = second eye cornea swelling
TECS = third eye cornea swelling
max(30min to 75min) = maximum swelling of the individual eye at 30 to 75 minutes max
(30min to 240min) = maximum swelling of the individual eye at 30 to 240 minutes
- Small negative numbers for swelling following application are counted as zero (larger negative numbers due to erosion invalidate the swelling evaluation, but indicate a severe effect)

- Cornea opacity was calculated according to the following formulae:
CO at time t = CO at time t – CO at t=0
Mean COmax = [FECOmax(30min to 240min)+ SECOmax(30min to 240min) + TECOmax(30min to 240min)] / 3
FECO = first eye cornea opacity
SECO = second eye cornea opacity
TECO= third eye cornea opacity max
(30min to 240min) = maximum opacity of the individual eye at 30 to 240 minutes

- Fluorescein retention was calculated according to the following formulae:
FR at time t = FR at time t – FR at t=0
Mean FR = [FEFR (30min) + SEFR(30min) + TEFR(30min)] / 3
FR = fluorescein retention
FEFR = first eye fluorescein retention
SEFR = second eye fluorescein retention
TEFR = third eye fluorescein retention

STORAGE OF CORNEAS
- At the end of the procedures, the corneas from the eyes were carefully removed from the eyes and placed individually into labelled containers of preservative fluid (10% neutral buffered formalin) for potential histopathology and stored.

Irritation parameter:

cornea opacity score

Run / experiment:

Mean

Value:

0

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: ICE Class I

Irritation parameter:

percent corneal swelling

Run / experiment:

Mean

Value:

1

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: ICE Class I

Irritation parameter:

fluorescein retention score

Run / experiment:

Mean

Value:

0.17

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: ICE Class I

Other effects / acceptance of results:

- Results of this in vitro eye irritation study, in isolated chicken eyes, suggest that the test material was not irritating.
- The positive control, imidazole, was classed as severely irritating, GHS Classification: Category 1.
- The negative control, sodium chloride 0.9%, had no significant effects on the chicken eyes in this study.

Table 1: Study Results

Observation	Test Material		Positive Control		Negative Control
	Value	ICE Class	Value	ICE Class	Value	ICE Class
Mean maximum corneal swelling at up to 75 min	1 %	I	11 %	II	1 %	I
Mean maximum corneal swelling at up to 240 min	1 %	I	12 %	II	1 %	I
Mean maximum corneal opacity	0.00	I	4.00	IV	0.00	I
Mean fluorescein retention	0.17	I	2.83	IV	0.00	I
Other Observations	Minimal test material was stuck on the cornea after the post-treatment rinse. The cornea surface was clear 240 min after the post-treatment rinse		The positive control material was stuck on the cornea surface after the post-treatment rinse. The cornea surface was not cleared 240 min after the post-treatment rinse.		None
Overall ICE Class	3 x I		1 x II, 2 x IV		3 x I

 

Interpretation of results:

other: Not classified in accordance with EU Criteria.

Conclusions:

Under the conditions of this study, the test material was not irritating to eyes.

Executive summary:

The eye irritation potential of the test material was investigated in accordance with the standardised guideline OECD 438, under GLP conditions.

An in vitro eye irritation study of the test material was performed in chicken’s eyes.

After the zero reference measurements, 30 μg of the test material was applied onto the centre of the cornea such that the entire surface of the cornea was covered. After 10 seconds, the surface was rinsed with saline. The positive control eyes were treated in a similar way with 30 μg imidazole. The negative control eye was treated with 30 μL of isotonic saline. The eyes were examined for a period of 4 hours following treatment.

The results suggested that the test material was not irritating. The positive control, imidazole, was classed as severely irritating, GHS Classification: Category 1. The negative control, sodium chloride 0.9%, had no significant effects on the chicken eye in this study.

Under the conditions of this study, the test material was not irritating to eyes.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin Irritation

The skin irritation potential of the test material was investigated in accordance with the standardised guideline OECD 439, under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

During the study disks of EPISKIN (three units / treatment) were treated with the test material and incubated for 15 minutes at room temperature. Exposure of test material was terminated by rinsing with PBS 1x solution (0.9%). Epidermis units were then incubated at 37°C for 42 hours. The viability of each disk was assessed by incubating the tissues for 3 hours with MTT solution. The formazan precipitated was then extracted using acidified isopropanol and quantified spectrophotometrically.

SDS 5% and PBS treated epidermis were used as positive and negative controls respectively. For each treated tissue viability was expressed as a % relative to negative control. The test material is considered to be irritant to skin, if the mean relative viability after 15 minutes exposure and 42 hours post incubation is less or equal (≤) to 50% of the negative control.

All validity criteria were within acceptable limits and therefore the study can be considered as valid. The test material treated tissues had a mean tissue viability of 93% after treatment.

Under the conditions of this study, the test material is not irritating to the skin.

Eye Irritation

The eye irritation potential of the test material was investigated in accordance with the standardised guideline OECD 438, under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

An in vitro eye irritation study of the test material was performed in chicken’s eyes.

After the zero reference measurements, 30 μg of the test material was applied onto the centre of the cornea such that the entire surface of the cornea was covered. After 10 seconds, the surface was rinsed with saline. The positive control eyes were treated in a similar way with 30 μg of imidazole. The negative control eye was treated with 30 μL of isotonic saline. The eyes were examined for a period of 4 hours following treatment.

The results suggested that the test material was not irritating. The positive control, imidazole, was classed as severely irritating, GHS Classification: Category 1. The negative control, sodium chloride 0.9%, had no significant effects on the chicken eye in this study.

Under the conditions of this study, the test material was not irritating to eyes.

Justification for classification or non-classification

In accordance with the criteria for classification as defined in Annex I, Regulation (EC) No 1272/2008, the substance does not require classification with respect to skin and eye corrosion or irritation.