Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
July 2017 - August 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
Batch Number: 21747297V0

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The test solutions were collected from the negative control, WSF control, and 100 mg/L test solution at test initiation, renewal, and at test completion.

Test solutions

Vehicle:
no
Details on test solutions:
The test item is a difficult item as defined by the OECD Guideline 23 (2000) because it is a multi-component substance with inconsistent water solubility. In order to maximise the amount of test item in solution, the test solutions were prepared as water soluble fractions (WSF) using the methodology described below.
One water soluble fraction (WSF) was prepared at 100 mg/L nominal loading rate and tested alongside a negative control, and a WSF control. Two sets of WSFs were prepared to create test solutions for test initiation and for the water renewal.
Each solution was stirred on a stirplate using a magnetic stir bar to create a vortex (~10%) and with plexiglass to avoid contamination and evaporation. Stirring occurred for approximately 20 hours at which time the solutions were drained through the side arm located at the bottom third of the aspirator bottle, discarding the first ~100 mL.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
In-house culture; original culture was obtained from Aquatic BioSystems (Fort Collins, CO) in April 2017 (identified by EcoAnalysts Inc. on March 10, 2016 as D. magna)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
80 - 110 mgL as CaCO3
Test temperature:
19.3 - 20.3 °C
pH:
7.7 - 8.3
Dissolved oxygen:
8.3 - 9.0 mg/L
Nominal and measured concentrations:
Nominal Loading Rate of the test item: 100 mg/L
Measured Loading Rates: 3.31 mg/L as Lithium, 1.42 mg/L as Iron, 14,76 mg/L as Phosphorus
Details on test conditions:
Test organisms per replicate: 5
Replicates: 4
Photo period: 16-hr light: 8-hr dark with a 30 minute transition period
Light intensity: 551 - 709 lux
Reference substance (positive control):
no
Remarks:
No reference item was employed. However, a reference item test using zinc sulphate heptahydrate (ZnSO4•7H2O, CAS# 7446-20-0) was conducted as a separate study.

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion