Registration Dossier
Registration Dossier
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EC number: 916-603-5 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
In the acute immobilization test withDaphnia magna, the effects of the test itemBasic Red 76were determined at the test facility according to OECD guideline 202.The study was conducted under semi-static conditions over a period of 48 hours with 5concentrations of the test item Basic Red 76 in the range of 6.25 to 100 mg/L, prepared in a geometric series with a separation factor of 2.All tested concentration levels were visually clear and exhibited a concentration-dependent red coloring.Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration leveland the control.The concentrations of the test item Basic Red 76 were analytically verified via HPLC-DAD in fresh media of all concentration levels and the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 97 to 107% of the nominal values and 89 to 106% in old media at renewal and at the end of the test (24 and 48 hours). Since the measured test item concentrations were within the range of ±20% of the nominal concentrations, the effect concentrations are based on the nominal concentrations of the test itemBasic Red 76.The validity criteria of the test guidelines were fulfilled. Based on the nominal concentrations of the test item Basic Red 76, the 48 hour EC50 for Daphnia magna was 26.6 mg/L (95% confidence limits : 15.1-47.2 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 26.6 mg/L
Additional information
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