Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl ethylphosphonate
EC Number:
201-111-9
EC Name:
Diethyl ethylphosphonate
Cas Number:
78-38-6
Molecular formula:
C6H15O3P
IUPAC Name:
diethyl ethylphosphonate
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Remarks:
Gas Chromatography (GC) with FID detection

Study design

Test type:
not specified
Water media type:
other: tap water
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 2ºC
pH:
7.87
Nominal and measured concentrations:
10 mg L-1, 100 mg L-1, 1000mg L-1
Details on test conditions:
Duration Three hours
Replicates One replicate/treatment (positive control)
One replicate for concentration (10 mg L-1)
One replicate for concentration (100 mg L-1)
Three replicates for concentration (1000 mg L-1)
Water Tap water
Feeding Nutrient solution, 16 mL/vessel
Temperature 20 ± 2ºC
Reference substance (positive control):
yes
Remarks:
Copper sulphate penta hydrate (mg) Chlorine-free tap water (mL) Synthetic sewage (mL) Activated sludge suspension (mL)

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance was solubilized in water because of its solubility. As there were no statistically significant toxic effects at the highest concentration (1000 mg L-1), no further testing was required at higher or lower concentrations as outlined in OECD 209, 2010.