Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl ethylphosphonate
EC Number:
201-111-9
EC Name:
Diethyl ethylphosphonate
Cas Number:
78-38-6
Molecular formula:
C6H15O3P
IUPAC Name:
diethyl phosphonate
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Remarks:
Gas Chromatography (GC) with FID detection

Study design

Test type:
not specified
Water media type:
other: tap water
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 2ºC
pH:
7.87
Nominal and measured concentrations:
10 mg L-1, 100 mg L-1, 1000mg L-1
Details on test conditions:
Duration Three hours
Replicates One replicate/treatment (positive control)
One replicate for concentration (10 mg L-1)
One replicate for concentration (100 mg L-1)
Three replicates for concentration (1000 mg L-1)
Water Tap water
Feeding Nutrient solution, 16 mL/vessel
Temperature 20 ± 2ºC
Reference substance (positive control):
yes
Remarks:
Copper sulphate penta hydrate (mg) Chlorine-free tap water (mL) Synthetic sewage (mL) Activated sludge suspension (mL)

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance was solubilized in water because of its solubility. As there were no statistically significant toxic effects at the highest concentration (1000 mg L-1), no further testing was required at higher or lower concentrations as outlined in OECD 209, 2010.