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EC number: 213-191-2
CAS number: 928-95-0
The capacity of Trans-hex-2-en-1-ol to induce gene mutation in bacteria
was evaluated during a GLP-compliant study performed in accordance with
the OECD Testing Guideline 471.
Bacterial strains were Salmonella typhimurium TA1537, TA98, TA1535 and
TA100, and Escherichia coli WP2uvrA. Experiments were performed with and
without metabolic activation using S9-mix.
Dimethyl sulphoxide was selected as a vehicle and used for negative
control, giving counts of revertant colonies within the normal range.
Relevant positive controls were selected for each bacteria strains in
accordance with the OECD Testing Guideline 471 and induced marked
increases in the frequency of revertant colonies, both with or without
metabolic activation. Therefore all the controls were considered as
Plate incorporation method (experiment 1) and pre-incubation method
(experiment 2) were used. The maximum dose level of the test item was
5,000 μg/plate in both experiments.
In experiment 1 the test item induced toxicity as weakened bacterial
background lawns and/or substantial reductions in the revertant colony
frequency of all of the Salmonella strains in both the presence and
absence of S9-mix at 5,000 μg/plate. No toxicity was noted to
Escherichia coli strain WP2uvrAin either the absence or presence of
S9-mix at any test item dose level.
In experiment 2 the test item again induced a toxic response with
weakened bacterial background lawns noted to all of the Salmonella
strains dosed in the absence of S9-mix at 5,000 μg/plate and to all of
the tester strains at the same dose concentration in the presence of
S9-mix. No toxicity was noted to Escherichia coli strain WP2uvrAdosed in
the absence of S9-mix at any test item dose level.
There were no increases in the frequency of revertant colonies recorded
for any of the bacterial strains, with any dose of the test item, either
with or without metabolic activation (S9-mix) in Experiment 1 (plate
incorporation method). Similarly, no toxicologically meaningful
increases in the frequency of revertant colonies were recorded for any
of the bacterial strains, with any dose of the test item, either with or
without metabolic activation (S9-mix) in Experiment 2 (pre-incubation
method). Small, statistically significant increases in TA1535 revertant
colony frequency were observed in the presence of S9-mix at 1500
μg/plate in the second mutation test. However, these responses were
within the in-house historical vehicle/untreated control values for the
bacterial strain and were, therefore, considered of no biological
It is therefore concluded thatTrans-hex-2-en-1-ol was not mutagenic
under the conditions of the test.
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