trans-hex-2-en-1-ol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 278/4
- Source (laboratory, culture collection): SAMS Limited
- Age of inoculum (at test initiation):
- Method of cultivation: culture were prepared from stock laboratory cultures by inoculating autoclaved freshwater at 120 °C for 15 minutes with nutrient medium (3N-BBM+V) 2 to 4 days before test commencement and incubated at approximately 21 +/- 2 °C under constant illumination as the inoculum source for subsequent toxicity tests.

ACCLIMATION
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: None reported

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

226 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

The 72-hour EC50 was 226 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Executive summary:

The short-term toxicity of Trans-hex-2-en-1-ol to algae was determined during a GLP-compliant study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

The 72-hour EC50 was 226 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N° 1272/2008.

Description of key information

The short-term toxicity of Trans-hex-2-en-1-ol to algae was determined during a GLP-compliant study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

The 72-hour EC50 was 226 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N° 1272/2008.

Key value for chemical safety assessment

EC50 for freshwater algae:

226 mg/L