2-hydroxy-3-methylcyclopent-2-enone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-3-methylcyclopent-2-enone

EC Number:

201-303-2

EC Name:

2-hydroxy-3-methylcyclopent-2-enone

Cas Number:

80-71-7

Molecular formula:

C6H8O2

IUPAC Name:

2-hydroxy-3-methylcyclopent-2-en-1-one

Specific details on test material used for the study:

The following information concerning identity and composition of the test item was pro-vided by the sponsor.
Name Methyl Cyclopentenolone
Batch no. D270001706002
Appearance white, crystal powder
Composition Methyl Cyclopentenolone
Purity >99.0%
Homogeneity homogeneous
Expiry date 07. Jun. 2018
Storage Room Temperature (20 ± 5°C)

The following additional information was relevant to the conduct of the study, according to
OECD 471:

CAS No. 80-71-7
EINECS-No. 201-303-2
Stability H2O: unknown; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Solubility H2O: < 0,1 g/L; EtOH: >1 g/L; acetone: >1 g/L; CH3CN: >1 g/l; DMSO: >1 g/l

Method

Species / strain

Species / strain / cell type:

other: TA97a TA98 TA100 TA102 TA1535

Test concentrations with justification for top dose:

5000 µg/plate

Vehicle / solvent:

Acetone

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Details on test system and experimental conditions:

All Salmonella typhimurium strains were obtained from TRINOVA BioChem GmbH (batch: TA97a: 4997D, TA98: 5011D, TA100: 4996D, TA102: 4982D, TA1535: 5012D) and were stored as lyophilizates in the refrigerator at 2-8 °C.
The lyophilizates were used to prepare permanent cultures which were filled into vials and stored at < - 75 °C.

Eight hours before the start of each experiment, an aliquot of a permanent culture per strain to be used was taken from the deep freezer to inoculate a culture vessel containing nutrient broth. After incubation overnight for eight hours at 37 ± 1 °C, the cultures were used in the experiment. During the test, the cultures were stored at room temperature as to prevent changes in the titre.

Results and discussion

Test results

Key result

Species / strain:

other: TA97a, TA98, TA100, TA102 and TA1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid