2-hydroxy-3-methylcyclopent-2-enone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-3-methylcyclopent-2-enone

EC Number:

201-303-2

EC Name:

2-hydroxy-3-methylcyclopent-2-enone

Cas Number:

80-71-7

Molecular formula:

C6H8O2

IUPAC Name:

2-hydroxy-3-methylcyclopent-2-en-1-one

Specific details on test material used for the study:

Test item: Methyl Cyclopentenolone
Common name of active ingredient (a.i): Methyl cyclopentenolone
CAS number of a.i: 80-71-7
Molecular weight of a.i.: 112.13 g.moL-1
Molecular formula of a.i.: C6H8O2
Received on: 12/Dec/2017
Bioagri Laboratórios Ltda. code: AGR-0921/17
Declared concentration of a.i. (Sponsor): >99.0% w/w
Batch number: D270001706002
Physical state: White, crystal powder
Manufacture date: 08/Jun/2017
Expiry date: 07/Jun/2018
Information source: Information provided by the sponsor

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Details on test solutions:

The primary stock solution used in the range-finding test was prepared by dissolving 0.025 g of the test substance in volume completed to 250 mL with OECD medium, resulting in a concentration of 100 mg.L-1. The stock solution of 10 mg.L-1 was prepared from dilution of the primary stock solution.
The stock solution used in the definitive test was prepared by dissolving 0.1 g of the test substance in volume completed to 1000 mL with OECD medium, resulting in a concentration of 100 mg.L-1.
From these initial stock solutions, a series of dilutions was prepared in order to obtain the desired tested concentrations

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Test System Selection Justification:
The alga species used as test system is recommended in the Guideline OECD 201 (2011).


Origin of the Test System:
The test system was obtained from Culture Collection of Algae at Goettingen University - SAG 61.81 on October, 08th, 2013, and is recommended by the Guideline OECD 201 (2011).


Stock Culture Maintenance:
The stock culture was prepared and maintained under lab-controlled conditions in solid medium with protease peptone.
The inoculated solid medium was kept inside a culture room, with controlled temperature of 21 to 24°C and luminosity of 2100 ± 10% lux. After algae cultures reached an ideal growth (about one week), they were maintained at temperature of 2 to 6°C and luminosity of 100 to 200 lux in order to slow down the algae growth.

Study design

Test type:

static

Total exposure duration:

72 h

Test conditions

Test temperature:

22.0 to 22.3oC

pH:

7.40-8.14

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

other: EyC50

Effect conc.:

3.44 mg/L

Basis for effect:

other: yield

Remarks on result:

other: (95% confidence limits = 2.75 to 4.29 mg.L-1)

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

30.05 mg/L

Basis for effect:

growth rate

Remarks on result:

other: (95% confidence limits = 22.32 to 40.46 mg.L-1)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes