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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From April 20, 2016 to October 25, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: National Chiayi University Freshwater Biological Resource Center- Source: National Chiayi University Freshwater Biological Resource Center
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Nominal and measured concentrations:
For Range-finding test: 0.16, 0.80, 4.00, 20.00 and 100.00 mg/L
For Limit test: 100.00 mg/L
Details on test conditions:
- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for limit test
- No. of vessels per control (replicates): 4 for limit test
- Photoperiod: 16-hr light: 8-hr dark
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

In range-finding test, the 0% of control group were immobilized and the dissolved oxygen concentration at the end of the test was 8.37-8.51 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The results of the range-finding test showed that EC50 was greater than 100.00 mg/L.

In limit test, the results showed below:

 

Table 1. The numbers immobilized for the limit test on day 1 and 2

Days

Group

(mg/L)

Numbers of no immobilized

Numbers of activity immobilized

Numbers of discoloration

Numbers of trapping at surface of water

Day 1

Control group

0.00

19

1

0

0

Test group

100.00

20

0

0

0

Day 2

Control group

0.00

19

1

0

0

Test group

100.00

20

0

0

0

Table 2. The percentages immobilized and EC50 for the limit test

Group

(mg/L)

Percentages immobilized (%)

EC50

(mg/L)

Day 0

Day 1

Day 2

Control group

0.00

0.00

5.00

5.00

Test group

100.00

0.00

0.00

0.00

>100.00

Table 3. Temperature, dissolved oxygen and pH value for the limit test

Group

(mg/L)

Day 0

Day 2

Temperature

()

Dissolved

oxygen

(mg/L)

pH

value

Temperature

()

Dissolved

oxygen

(mg/L)

pH

value

Control group

0.00

20.0

8.82 ± 0.07

7.60 ± 0.01

20.0

8.69 ± 0.10

7.48 ± 0.02

Test group

100.00

8.51 ± 0.15

7.63 ± 0.02

8.75 ± 0.03

7.49 ± 0.04

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of CR SB07 was >100 mg/L.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100059001EN which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CR SB07 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of CR SB07 was >100 mg/L.

Description of key information

The 48-hr EC50 of CR SB07 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

In both range-finding and limit test, not more than 10 % of the Daphnia were immobilized in the control group and the dissolved oxygen concentration at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of CR SB07 was >100 mg/L.