Reaction products of 2,4-bis(2-chlorophenyl)-5-(3,4-dimethoxy phenyl)-1H-imidazole and 2-(2-chlorophenyl)-4,5-diphenyl-1H-imidazole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Plant drainage testing method, acute toxicity test of fish and shellfish (71. of JIS K 0102-1998).

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Purity: 100% as a reaction product

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
Dispersant: HCO-40
The provided samples and HCO-40 at 20 times its amount were dissolved in acetone, and after distilling the acetone, ion-exchange water was added, so that a stock solution with a test substance concentration of 1000 mg/L was prepared. The operation was carried out under a light-shielded condition.

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

Kind of fish: Rice fish, Medaka, Oryzias latipes
Reason for selection: Since the sensitivity is similar to that of carp and it is easily obtained as a fish for testing.
Supply source: Ogawa Shoten
Cumulative culture condition: Upon receipt, abnormal fish were removed, and the remaining fish were treated with a medication in a cumulative culture tank and held for 12 days in a flow-water state.
Domestication condition: After the cumulative culture, the fish were transferred to a domestication tank, treated with a medication, and domesticated. During the domestication, abnormal fish were removed, and the remaining fish were held for 15 days in a flow-water state at a water temperature of 25 ± 2ºC. Then, the fish were sorted, treated with a drug, and held for 38 days under flow-through conditions.
Weight: Average 0.22 g
Total length: Average 3.0 cm
Sensitivity test: The 48-h LC50 value of a reference material PCP-Na (pentachlorophenol sodium reagent using the test fish of the same lot was 0.420 mg/L.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

24.1ºC

pH:

7.9

Dissolved oxygen:

At time of exposure start 7.9 mg/L
Before water exchange 6.5 mg/L

Nominal and measured concentrations:

250 mg/L

Details on test conditions:

Number of provided fish: 10 fish/concentration level
Exposure period: 96 h
Exposure method: Half water cut-off type (water exchange every 8-16 h) m-- static renewal
Illumination: Under a light-shielded condition for the exposure period.

Reference substance (positive control):

yes

Remarks:

Pentachlorophenol sodium reagent

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 250 mg/L

Details on results:

96-h LC50 value of the test substance > 250 mg/L
96-h LC50 value was calculated by the Doudoroff method.
The concentration of the dispersant (HCO-40) used at that time was 5000 mg/L and the 96-h LC50 value of the dispersant was 50000 mg/L, the test at a concentration higher than that was not carried out in consideration of the influence of the toxicity of the dispersant.

Applicant's summary and conclusion

Conclusions:

96-h LC50 was > 250 mg/L

Executive summary:

This OECD 210 study with Oryzias latipes was part of a BCF study with Carp. The 96-h LC50 was determined to be > 250 mg/L.