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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

The test item was evaluated for the ready biological degradability in the Closed Bottle Test, according to the EU Method C.4E (2008) and the OECD Guideline 301D (1992). The test item was sufficiently soluble in used mineral medium so the dosage from the stock solution was carried out. Sodium benzoate was used as a reference substance. The dosage was made up from the stock solution. In parallel to the main test, the toxicity test was performed.

The test item degraded by 8.0 % over 28 days. The reference substance degraded by 72.4 % over the same period. As the test item contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out. The test was performed at temperature of 20 ± 1 °C with pH values of 7 to 8 in solution at the beginning of the test. The calculations for biological degradation are based on ThOD (Theoretical Oxygen Demand) values of the test item and reference substance. The prescribed validity criteria in the test were fulfilled and the test item did not inhibit the inoculum used. Since all criteria of acceptability were met, this study is considered valid.