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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 - 27 Aug 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Qualifier:
according to
Guideline:
other: EG Guideline 92/69/EWG
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: waterflea
- Strain: Klon 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): neonates
- Method of breeding: isolation of parent daphnids approximately 24 h prior to test start.
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 +/- 1 °C
pH:
7.3 - 7.5
Dissolved oxygen:
7.2 - 8.6 mg/L
Nominal and measured concentrations:
nominal: 5, 9, 15, 26, 45, 80 and 135 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: round bottom glasses
- Material, size, headspace, fill volume: filled with 10 mL test medium
- Aeration: none
- No. of organisms per vessel: 5 (20 animals per concentration)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium according to Elendt
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test end

Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
Details on results:
48h EC50 based on a-WAS = 7.2 mg/L

Concentration [mg/L]

Test duration

24 h

48 h

Number mobile

Number immobile

% immobile

Number mobile

Number immobile

% immobile

Control

20

0

0

20

0

0

5

20

0

0

20

0

0

9

19

1

5

4

16

80

15

7

13

65

0

20

100

26

0

20

100

0

20

100

45

0

20

100

0

20

100

80

0

20

100

0

20

100

135

0

20

100

0

20

100

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
27 - 29 Sep 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: waterflea
- Strain: clone 5
- Source: in-house culture
- Age at study initiation: approximately 24 h
- Method of breeding: for breeding, females are maintained in M4 medium in 1 L beakers.
- Feeding during test: none


ACCLIMATION
- Acclimation conditions (same as test or not): M4 medium in 1 L beakers
- Type and amount of food: the amount of food, comprising Scenedesmus subspicatus, is such that the amount added is consumed by the animals.
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 +/- 1 °C
pH:
7.7 - 7.8
Dissolved oxygen:
7.9 - 8.1 % (there may be a mistake in the study) - mg/L instead of %
Nominal and measured concentrations:
nominal: 0.4, 0.7, 1.2, 2.0, 3.5, 6.0 and 10 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size: round-bottomed test tubes calibrated to 10 mL
- Aeration: none
- No. of organisms per vessel: 5 (+/-1)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water: 294 mg/L CaCl2 x 2H2O, 123 mg/L MgSO4 x 7 H2O, 63 mg/L NaHCO3, 5.5 mg/L KCl
- Ca/Mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: darkness

Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

 

Concentration
[mg/L]

Test period (h)

24 h

48 h

Immobility [%]

Immobility [%]

Control

5

5

0.4

0

0

0.7*

5

30

1.2

5

0

2.0

0

0

3.5

0

5

6.0

0

0

10

40

100

*not used for evaluation, because no effect could be

observed in case the next following concentration

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Reason / purpose:
read-across source
GLP compliance:
yes
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
Remarks:
Source, key, 68891-38-3, 1993b
Details on results:
48h EC50 based on a-WAS = 7.2 mg/L

Description of key information

EC50 (48 h) for Daphnia magna = 7.4 mg a.i./L (nominal, OECD 202) 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
7.4 mg/L

Additional information

No acute invertebrate studies on toxic effects of AES (C12-14, 1-2.5 EO) TEA (CAS 157627-94-6) are available. Therefore, a read-across approach from two structural similar AES (C12-14, 2.5EO) mixtures was applied.

Both studies were conducted according to OECD guideline 202 and GLP with Daphnia magna as test species. In one study the daphnids were exposed for 48 hours to AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3), which resulted in an EC50 value of 7.4 mg a.i./L (nominal) (Sasol, 1993b). In the second study the test organisms were exposed for 48 hours to AES (C12-14, 1-2.5EO) MIPA (CAS 1187742-72-8), which resulted in an EC50 value of 7.7 mg test item/L (nominal) (Sasol, 1999b).