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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 - 16 Sep 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
other: EG Guideline 92/69 C.1
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3 cm +/- 0.5 cm
- Weight at study initiation (mean and range, SD): approximately 0.25 g
- Feeding during test
- Food type: no feeding


ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food: TetraMin at a concentration of 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): <= 5% seven days prior test until test start

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
11 ° dH
Test temperature:
19 °C - 20 °C
pH:
8.0 - 8.2
Dissolved oxygen:
80% - 99%
Nominal and measured concentrations:
nominal: 4.3, 6.0, 8.5, 11.8 and 17.1
mean measured (over 96 h): 4.6, 6.8, 13.9, 12.1 and 17.2
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: approximately 45 L aquaria
- Aeration: continuously
- Renewal rate of test solution (frequency/flow rate): approximately 10 L/h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 10



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water - Gelsenwasser AG


OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h darkness

Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
10 - 14 Nov 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg
- Length at study initiation (mean, SD): 3 cm +/- 0.5 cm
- Weight at study initiation (mean): approximately 0.26 g
- Feeding during test: none



ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): maintained in dechlorinated drinking water in 200 L tanks of in 300 L glass tanks at 20 +/- 2 °C with continuous flow.
- Type and amount of food: Trouvit at an amount of approximately 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): the mortality was 7 days before the test start <= 5%

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
approximately 11 °dH
Test temperature:
20 °C - 23 °C
pH:
8.1 - 8.2
Dissolved oxygen:
90% - 103%
Nominal and measured concentrations:
nominal: 2.1, 3.5, 6.0, 10.0 and 17.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 45 L tanks
- Aeration: continuous in the test tanks
- Renewal rate of test solution (frequency/flow rate): flow rate: 10 L/h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water (Gelsenwasser AG)


OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h darkness
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Concentration
[mg/L]

Test period (h)

24 h

48 h

72 h

96 h

Mortality [%]

Mortality [%]

Mortality [%]

Mortality [%]

Control

0

0

0

0

2.1

0

0

0

0

3.5

0

0

0

0

6.0

0

0

0

0

10.0

100

100

100

100

17.0

100

100

100

100

Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Reason / purpose:
read-across source
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Source, key, 68891-38-3, 1994

Description of key information

 LC50 (96 h) for Danio rerio = 7.1 mg a.i./L (nominal, OECD 203)

Key value for chemical safety assessment

LC50 for freshwater fish:
7.1 mg/L

Additional information

No acute fish study on toxic effects of AES (C12-14, 1-2.5 EO) TEA (CAS 157627-94-6) is available. Therefore, a read-across approach from two structural similar AES (C12 -14, 2.5EO) mixtures was applied.

Both studies were conducted under flow-through conditions according to OECD guideline 203 and GLP with the zebra fish Danio rerio as test species. In one study the fish were exposed for 96 hours to AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3), which resulted in a LC50 value of 7.1 mg a.i./L (nominal) (Sasol, 1994). In the second study the fish were exposed for 96 hours to AES (C12-14, 1-2.5EO) MIPA (CAS 1187742 -72 -8), which resulted in a LC50 value of 7.7 mg a.i./L (nominal) (Sasol, 1999a).