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EC number: 815-500-1 | CAS number: 1853175-99-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
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- Dissociation constant
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/07/2015-31/07/2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,3-dioxane, 2-(3,3-dimethyl-1-cyclohexen-1-yl)-2,5,5-trimethyl
- Cas Number:
- 1853175-99-1
- Molecular formula:
- C15H26O2
- IUPAC Name:
- 1,3-dioxane, 2-(3,3-dimethyl-1-cyclohexen-1-yl)-2,5,5-trimethyl
Constituent 1
- Specific details on test material used for the study:
- Received on 007/15 from the sponsor. It presented as a colourless translucent liquid.
Batch number: 100026324
97.5% for isomer 1 of Maderal
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- acetone
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Source: Phytosafe s.a.r.l., 2 rue Mar Dormoy, 64000 PAU, France
Age : Less than 24h at test initiation
Fed with living celles of green algaes
Adult females were transferred in a new volume before the test initiation. Young daphnids produced within 24h were used for the tests.
Daphnids not fed during the exposure period.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.4°C and kepr constant at +/- 1°C for both range-finding and definitive test
- pH:
- Water control : between 7.9-7.7
Solvent control : between 7.7
Maderal : between 7.9-7.6 in all tests - Dissolved oxygen:
- Water control : between 8.4-7.8 mg/L
Solvent control : between 8.4-7.7 mg/L
Maderal : between 8.4-7.6 mg/L in all tests - Nominal and measured concentrations:
- Nominal concentrations:
Range finding test: 0.002 ; 0.02 ; 0.2 ; 2.1 ; 20.6 mg/L
Definitive test: 0.6 ; 1.2 ; 2.5 ; 5.1 ; 10.3 mg/L - Details on test conditions:
- Test medium: M4 medium freshly reconstituted from mineral stock solutions.
Use of a Water control and a solvent control (actetone)
Tubes maintained in the dark in a climatic chamber - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, CAS n°7778-50-9, AnalaR Prolabo Ref. 26784.231, batch 07J050002
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50-24h value is between 0.6-2.1 mg/L. Calculations gave 1.33 mg/L as the measured value.
The results fulfilled the validity criteria base on Phytosafe historical data. - Reported statistics and error estimates:
- Effect pf Maderal, 95% confidence intervals
T0+24h : 1.0-6.7 mg/L
T0+48h : 1.0-4.5 mg/L
Effect of Potassium dichromate (95% confidence intervals)
T0+24h : 0.93-1.90 mg/L
Any other information on results incl. tables
Analytical verification: test item concentrations were maintained within 80 -120% of the nominal values over the test period.
Percentage of immobilization:
Water/Solvent control: 0% in every replicates
Other observations:
Initial / final pH similar between the treatment test and both control test
Final oxygen > 3mg/L in every cases
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The Short-term toxicity to aquatic invertebrates test was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).
The results are the following:
NOEC=1.2mg/L
EC50=2.2mg/L
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