Linseed oil, polymerized

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 March 2010 - 12 March 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Linseed oil, polymerized

EC Number:

614-114-9

Cas Number:

67746-08-1

Molecular formula:

Not applicable for UVCB

IUPAC Name:

Linseed oil, polymerized

Details on test material:

- Name of test material (as cited in study report): Standolized linseed oil
- Substance type: Hazy yellow/brown viscous liquid
- Physical state: liquid
- Analytical purity: treated as 100% pure
- Lot/batch No.: 09.317.013
- Expiration date of the lot/batch: 21 December 2010
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark
- Other: -

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: TOC-analysis from the WAF prepared at a loading rate of 100 mg/l and the untreated control
- Sampling method:
Frequency at t=0 h and t=48 h
Volume 40 ml
Storage Samples were stored in a refrigerator until analysis

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Preparation of the test solutions started with loading rates prepared individually at 1.0, 10 and 100 mg/l applying 48 hours of magnetic stirring to achieve maximum dissolution in test medium. The magnetic stirring was followed by a settlement period of 2 hours. This resulted in clear and colourless solutions with a floating layer. The Water Accommodated Fractions (WAFs) were then collected by siphoning the water phase. The final test solutions were all clear and colourless

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
-Common name: Daphnia magna
- Source: In-house laboratory culture
- Age at study initiation: < 24 hours
-Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

The hardness: 180 mg/l expressed as CaCO3

Test temperature:

between 19.2 and 20.3°C

pH:

At t=0 h: 7.7-7.8
At t=48 h:7.9

Dissolved oxygen:

At t=0 h: 8.9-9.6
At t=48 h:8.9-9.0

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentrations: WAFs prepared at loading rates of 1.0, 10 and 100 mg/l
Measured concentration: During the test samples for TOC-analysis were taken from the WAF prepared at a loading rate of 100 mg/l and the untreated control. The TOC concentrations measured in the treated samples were only slightly exceeding those measured in the control samples, indicating that the actual test substance concentration was very low. The actual measured concentration was at approximately 0.6 mg C/l when corrected for the carbon measured in the control samples. The TOC content of Standolized linseed oil was determined to be 77.88% . Thus, the actual Standolized linseed oil concentration in solution was approximately 0.8 mg/l.

Details on test conditions:

Test vessel: 100 ml, all glass
- Type (delete if not applicable): open - ~80 ml,
- Material, size, headspace, fill volume: glass, 100 ml, normal headspace
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for the highest concentration, 2 in the lower test concentrations
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium
- Culture medium different from test medium: yes, for culturing M7 medium is used


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Test concentrations: WAFs prepared at loading rates of 1.0, 10 and 100 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: > WAF prepared at 100 mg/l

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: > WAF prepared at 100 mg/l

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: WAF prepared at 100 mg/l

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: TOC

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: TOC

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: TOC

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: no mortility observed in control
- EC50/LC50:
The 24h-EC50 was 0.75 mg/l with a 95% confidence interval between 0.56 and 1.0 mg/l.
The 48h-EC50 was 0.36 mg/l with a 95% confidence interval between 0.28 and 0.49 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study Standolized linseed oil did not induce acute immobilisation of Daphnia magna when exposed to a WAF prepared at a loading rate of 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 exceeded a WAF prepared at a loading rate of 100 mg/l, which can be considered to represent the maximum soluble concentration in test medium. TOC-analyses showed that the actual concentration in solution approximated 0.8 mg/l.