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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Simplified study.
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 01JAT1272
- Expiration date of the lot/batch: no data
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at 4°C.
- Stability under test conditions: yes
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: 0, 1, 3.5, 10, 35, 60 and 100 mg/kg.
- Sampling method: On the day of the test, a stock solution at 100 mg / kg was made in the test medium. Concentrations of 60, 35, 10, 3.5 and 1 mg / kg were obtained by dilutions of the stock solution. Four vials were prepared for each concentration.
- Sample storage conditions before analysis: The test was carried out in temperature-controlled rooms (temperature between 18 ° C and 22 ° C at ± 1 ° C).
Incubation was done in the dark.
Vehicle:
no
Test organisms (species):
other: Daphnia similis
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia similis (Cladocera crustacea)
- Strain: CETESB
- Source: breeding of the ecotoxicity laboratory of the Paulinia - Rhodia Poliamida - Brazil Research Center.

ACCLIMATION
- Acclimation period: 24 hours

Test type:
static
Water media type:
freshwater
Remarks:
obtained from a natural source in the state of São Paulo (Ribeirão Pirai-Salto), indicated and controlled by the local Drire (CETESB).
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None.
Hardness:
no data
Test temperature:
18-22°C
pH:
7,2 - 7,6
Dissolved oxygen:
No data
Salinity:
no data
Conductivity:
no data
Nominal and measured concentrations:
100 mg/L (nominal concentration).
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 0

- Intervals of water quality measurement:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation.

VEHICLE CONTROL PERFORMED: yes
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: no
- Observations on body length and weight: no data
- Other biological observations: no data
- Mortality of control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
- Results with reference substance valid: yes . EC 50/24 hours: 0.25 mg/kg.
Validity criteria fulfilled:
yes
Conclusions:
Under the test conditions of this study, Vanillic acid is not harmful to Daphnids.
Executive summary:

The purpose of the study was to estimate the acute toxicity (EC50) of Vanillic acid to Daphnia (Daphnia similis) under static test conditions (Screening test, Reliability 2, Not GLP). The nominal concentration tested was 100 mg/L.

No immobilised Daphnia were observed during the study, therefore the 48-hour EC50 value was > 100 mg/L and the No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. In these test conditions, Vanillic acid is not harmful to Daphnids.

Description of key information

One study was available and considered as the key study. The purpose of this study was to estimate the acute toxicity (EC50) of Vanillic acid to Daphnia (Daphnia similis) under static test conditions (Screening test, Reliability 2, Not GLP). The nominal concentration tested was 100 mg/L.

No immobilised Daphnia were observed during the study, therefore the 48-hour EC50 value was > 100 mg/L and the No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. In these test conditions, Vanillic acid is not harmful to Daphnids.

Key value for chemical safety assessment

Additional information