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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The WAF of the test substance was not toxic to rainbow trout, Oncorhynchus mykiss, at the tested concentration. The 96 hour median lethal concentration (LC50) of the test material to rainbow trout was greater than 1,000 mg/L (expressed as the nominal amount of test substance used to prepare the WAF) and the no observed effect concentration (NOEC) was 1,000 mg/L. 
Nominal concentrations: 24, 48, 72 and 96-h LL50 (reported as “LC50” in the report) >1,000 mg/L. This is equivalent to 96-h LL0 (reported as “NOEC” in the report) = 1,000 mg/L.
Test results reported in the study as “lethal concentrations” are reported in this summary as “lethal loading”, because test results are based on WAF loading rates.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Marine water fish

Marine water fish
Effect concentration:
10 g/L

Additional information

In the key study, the acute toxicity of the water accommodated fraction (WAF) of a 1,000 mg/L mixture of the test material and water to the rainbow trout, Oncorhynchus mykiss, was investigated during a study conducted at T.R. Wilbury Laboratories, Inc. The test, which was designed to determine the toxicity of the WAF of the test substance, was performed from December 9 to 13, 1996 and was conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test) and GLP.

This study is presented as the key information as the reliability rating for this study is 1, according to the criteria of Klimisch, 1997, and this study was conducted most recently and was therefore chosen as the most reliable study.

There are two supporting studies available:

- The T.J. Ward, 1993 study (T.R. Wilbury Laboratories, Inc., report number: 9176-CMA/ESI) was not considered the key study as it was conducted less recently than the above key study. The study was conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test) and GLP and was considered to have a reliability rating of 1, according to the criteria of Klimisch, 1997.

Nominal concentrations: 96-h LL50 (reported as “LC50” in the report) >1,000 mg/L. This is equivalent to 96-h LL0 (reported as “NOEC” in the report) of 1,000 mg/L.

Test results reported in the study as “lethal concentrations” are reported in this summary as “lethal loading rate”, because test results are based on WAF loading rates.

- Supporting information, obtained from the 2000 European Chemicals Bureau IUCLID Data Set for the substance is presented, however this is secondary source information and is considered to have a reliability rating of 4, according to the criteria of Klimisch, 1997.

Marine conditions:

- The Nicholson R.B. and Breteler R.J. 1986 study (Springborn Bionomics, Inc. report number: BW-86-04-1981) was not considered the key study for this endpoint as it was conducted less recently than the above key study and also it was conducted using seawater and a fish native to brackish waters as opposed to the more typical freshwater conditions used in testing for this endpoint. However for chemical safety assessment purposes, the LC50 from this study will satisfactory fulfil the marine key study endpoint.

The study was conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test) and GLP and was considered to have a reliability rating of 1, according to the criteria of Klimisch, 1997.

The 96-hour mortality of the fish tested at 100% Water Soluble Fraction (WSF) was 5% Based on this result, no Tier II toxicity test was necessary. The LC50 can therefore be consired to be >10 g/l.