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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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activated sludge respiration inhibition testing
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
Justification for type of information:
As the target substance hydrolyses rapidly (half-life < 30 minutes) the intrinsic properties are related to hydrolysis products of the target substance. This information is used as a supporting evidence on the toxicity of the target substance in CSA.
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
according to guideline
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
not specified
Analytical monitoring:
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Source: Municipal wastewater treatment plant.
Test type:
Water media type:
Limit test:
Total exposure duration:
3 h
Key result
3 h
Dose descriptor:
Effect conc.:
> 1 000 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

No studies were conducted on the target substance, Tetrakis [[2,2',2"-nitrilotris[ethanolato]](1-)-N,O]zirconium. As the target substance hydrolyses rapidly (half-life < 30 minutes) the intrinsic properties are related to hydrolysis products of the target substance.

On the basis that if entering the environmental, this substance will quickly dissociate to form the inorganic (poorly soluble) zirconium dioxide and 2,2',2''-Nitrilotriethanol (triethanolamine). The zirconium dioxide is not considered further in this report as aquatic toxicity assessments do not apply.

Short-term toxicity studies of triethanolamine are well-investigated:

Fish: LC50 (96h), Pimephales promelas, was 11800 mg/l (Geiger, D.L. et al., 1985)

Invertebrates: EC50 (24h) Daphnia magna, was 1850 mg/l (Bringmann 1982)

Algae or cyanobacteria: EC50 (72h),Desmodesmus subspicatus, was 512 mg/l (Aman and Steinhaeuser, 1986)

Microorganisms: IC50 (3h), activated sludge, > 1000 mg/l (Klecka, et al. 1985).