Registration Dossier
Registration Dossier
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EC number: 203-026-2 | CAS number: 102-36-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well documented and scientifically acceptable
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,4-dichlorophenyl isocyanate
- EC Number:
- 203-026-2
- EC Name:
- 3,4-dichlorophenyl isocyanate
- Cas Number:
- 102-36-3
- Molecular formula:
- C7H3Cl2NO
- IUPAC Name:
- 1,2-dichloro-4-isocyanatobenzene
- Details on test material:
- no data
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 0.0032 µg/plate up to 4.0 µg/plate
- Vehicle / solvent:
- no data
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- not further specified
Results and discussion
Test results
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The test item did not cause gene mutations; therefore, the test item is considered to be non-mutagenic in this bacterial reverse mutation assay.
- Executive summary:
Method
Ames test with E.coli WP 2 uvr A with and without metabolic activation
Result
negative
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