Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 March 2017 - 26 April 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
Version / remarks:
2004
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 830.7570 (Partition Coefficient, n-octanol / H2O, Estimation by Liquid Chromatography)
Version / remarks:
1996
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
Appearance: off-white powder
Test item storage: at room temperature

Results and discussion

Partition coefficientopen allclose all
Key result
Type:
log Pow
Partition coefficient:
6.1
Temp.:
20 °C
Remarks on result:
other: Peak area = 96%. Unbuffered mobile phase.
Type:
log Pow
Partition coefficient:
5.5
Temp.:
20 °C
Remarks on result:
other: Peak area= 4.1%.
Details on results:
- Retention times of reference substances used for calibration: see table below
- Details of fitted regression line: log k' = 0.292 x log Pow – 0.633 (r = 0.998, n=12)
- Graph of regression line and chromatograms: see attachment
- The chromatogram of the test substance showed one major peak (96% area) and one small (4.1% area) peak. The log Pow for the major peak was reported as the log Pow of the substance.
- Average retention data for test item: see table below
- The value of log Pow obtained from duplicate measurements was within ± 0.1 log units.

Any other information on results incl. tables

Table: Results Partition coefficient n-octanol-water (HPLC method)

 

Substance

tr,1

[min]

tr,2

[min]

mean tr

(n=2)

log Pow

Pow

Area

%

Formamide (t0)

0.586

0.586

0.586

 

 

 

Biphenyl

2.487

2.490

 

4.0

 

 

1,2,4-Trichlorobenzene

2.861

2.867

 

4.2

 

 

Dibenzyl

4.099

4.115

 

4.8

 

 

Triphenylamine

7.488

7.519

 

5.7

 

 

4,4’-DDT

10.708

10.862

 

6.5

 

 

Benzo[ghi]perylene

17.353

17.532

 

7.2

 

 

Test item – peak 1

5.929

5.970

5.950

5.5

2.9E05

4.1

Test item – peak 2

8.922

8.998

8.960

6.1

1.4E06

96

Remark: the log Pow values of the reference substances are according to OECD TG 117.

Applicant's summary and conclusion

Conclusions:
The log Pow of the substance was determined to be 6.1.

Executive summary:

The log Pow of the substance was determined using the HPLC method in a GLP study according to EC A.8, OECD 117 and OPPTS 830.7570. The mobile phase was 75/25 (v/v) methanol/water and the wavelength of detection was 210 nm.

Formamide was used as unretained substance. Duplicate injections of unretained substance, reference substances and test item. The chromatogram of the test substance showed one major peak (96% area) and one small (4.1% area) peak. The log Pow of the substance (for the peak with area of 96%) was determined to be 6.1. The log Pow value for the peak with area of 4.1% was determined to be 5.5.