4-trans-(4-trans-Propylcyclohexyl)-cyclohexancarbonic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

1996-07-30 to 1996-09-03

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

yes

Remarks:

Only 3 test substance concentrations were applied, instead of at least 5 according to the guideline. No LC50 was determined.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Test solutions were prepared by stirring for 24 hours and then Ultra-Turrax treatment for 60 sec. Undissolved parts were removed by filtration (7-12 µm).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Name in test report: Zebrabärbling (Brachydanio rerio HAMILTON BUCHANAN)
- Source: Charles River
- Age at study initiation: approx. 3 months
- Length at study initiation: 2.5 - 3.5 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21.6 - 22.5°C

pH:

7.8 - 8.2

Dissolved oxygen:

7.7 - 9.2 mg/L (90.8 - 108.0 % of saturation)
1 extreme value: at 2.1 mg/L after 48 h 6.6 mg/L (77.9 % satur.)

Nominal and measured concentrations:

Nominal Concentration: 0.4, 0.9 and 2.1 mg/L; no measured concentration

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria 300 x 135 x 200 mm
- Fill volume: 5 L
- Aeration: yes
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to appendix 1 of EU test guideline

OTHER TEST CONDITIONS
- Photoperiod:16 h light / 8 h dark

TEST CONCENTRATIONS
- nominal concentrations: 0.4, 0.9, 2.1 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

other:

Remarks:

geometric mean LC0 and LC100

Effect conc.:

0.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 0.4 mg/L 1 fish (10 %) died. When the test was repeated, all test animals survived.

Validity criteria fulfilled:

no

Conclusions:

The geometric mean of LC0 and LC100 was calculated to be 0.9 mg/L.

Executive summary:

A test on acute toxicity to fish was conducted according to EU Guideline C.1.3. The test substance was applied at 3 concentrations instead of at least 5 as stipulated in the test guideline. Therefore, the test is considered to be not valid. Three test substance concentrations and one control were applied. The LC₀ was determined to be 0.4 mg/L and the LC₁₀₀ was determined to be 2.1 mg/L, respectively. The geometric mean of LC₀ and LC₁₀₀ was calculated to be 0.9 mg/L.

Description of key information

The geometric mean of LC₀ and LC₁₀₀ was calculated to be 0.9 mg/L. The short-term toxicity to fish study was disregarded due to major methodical deficiencies (reference 6.1.1-1).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.9 mg/L

Additional information

A test on acute toxicity to fish was conducted according to EU Guideline C.1.3. The test substance was applied at 3 concentrations instead of at least 5 as stipulated in the test guideline. Therefore, the test is considered to be not valid. Three test substance concentrations and one control were applied. The LC₀ was determined to be 0.4 mg/L and the LC₁₀₀ was determined to be 2.1 mg/L, respectively. The geometric mean of LC₀ and LC₁₀₀ was calculated to be 0.9 mg/L.