Copper, diamminebis(nitrato-kO)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Copper, diamminebis(nitrato-kO)

EC Number:

604-942-9

Cas Number:

15414-04-7

Molecular formula:

CuH6N4O6

IUPAC Name:

Copper, diamminebis(nitrato-kO)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling for analyses has been performed every 24 hours for each test concentration and for the control.

Test solutions

Vehicle:

yes

Remarks:

dilution water

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus 1820 (Crustacea Cladocera) coming from a continuous and balanced breeding carried out in the test facility, aged less than 24 hours at the start of the test.

Study design

Test type:

not specified

Water media type:

other: reconstituted water (dilution water)

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

273.6 mg/L expressed as CaCO3

Test temperature:

between 19.1 and 20.4°C

pH:

7.8

Dissolved oxygen:

8.6 mg/L

Conductivity:

648 µS/cm

Nominal and measured concentrations:

0.35 – 0.60 – 1.02 – 1.73 – 2.94 - 5.00 mg/L (nominal concentrations)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.97 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50 were calculated using a logistic regression analysis with a bootstrap estimation of the 95% intervals.
EC50 24h = 2.72 mg/L (2.13 – 2.76 mg/L) (nominal concentration)
EC50 48h = 1.97 mg/L (1.81 – 2.09 mg/L) (nominal concentration), corresponding to an exposure to a mixture of 0.0132 mg/L dissolved copper and 0.45 mg/L guanidine.

Results with reference substance (positive control):

Sensitivity of the test organism was checked using potassium dichromate. The EC50 24 hours was calculated using a logistic regression analysis with a bootstrap estimation of the 95% intervals.
EC50 24h: 1.12 mg/L (1.08 – 1.17 mg/L).
The EC50 24h is in the recommended range: 0.6 – 2.1 mg/L. This result confirms the appropriate sensitivity of the strain of Daphnia magna.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The analyses performed have shown that dissolved copper concentrations, even if they were low, were in a range that may affect the mobility of Daphnia magna. It was therefore impossible to identify which compound of test item MNP-363 was responsible for the observed effects.
Consequently, EC50 value have been expressed in concentrations of the mixture of copper (dissolved) and guanidine:
EC50 48h = 1.97 mg/L (1.81 – 2.09 mg/L) (nominal concentration), corresponding to an exposure to a mixture of 0.0132 mg/L dissolved copper and 0.45 mg/L guanidine.