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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27th April 2018 to 29 June 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 23 (Series on testing and assessment)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of butyl palmitate and butyl oleate and butyl (9Z,12Z)-octadeca-9,12-dienoate and 482-680-2
EC Number:
944-892-8
Molecular formula:
not applicable
IUPAC Name:
Reaction mass of butyl palmitate and butyl oleate and butyl (9Z,12Z)-octadeca-9,12-dienoate and 482-680-2
Test material form:
liquid: viscous

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Seven replicates of test item at 20 mg/L loading rate were inoculated at 5 017 cells per mL. A eight replicate non inoculated was used as colour control. A control (6 replicates) was tested under the same conditions with test media only.

Test solutions

Vehicle:
no
Details on test solutions:
the test item solution was prepared by dissolution of 20.0 mg of butyl avocadate distillated in 1 000 mL of the test medium to obtain a nominal concentration at 20 mg/L (experimental concentration = 20 mg/L). The solution was prepared in 2 L glass bottle and shaken for about 24h. Then this preparation was filtered through 0.2 µm filter (Whatman cellulose nitrate membrane filters). This solution was used for exposure and identified as saturation concentration.
The pH of this resulting solution was 7.8.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
Culture conditions :
Temperature: 23°C +/- 2°C
Light: continuous, with plant growth specific spectra fluorscent bulb, between about 4440 and 8880 Lux.
Freshwter (reconstituted from salts)
The algae are maintained at exponential growth rate by adding aliquots of algal culture to fresh reconstituted water samples weekly.
Origin: Culture at SGS France Rouen Laboratory, strain from SAG Gottingen collection (ID n°61.81).

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
23°C +/- 2°C
Dissolved oxygen:
Test medium solution pH was between 5.8 and 9.2 considred as acceptable for the wellbeing of the test organisms
pH Variation in control solutions (pH units) <+/- 1.5
Nominal and measured concentrations:
experimental concentration
Details on test conditions:
The test was conducted in 250 mL glass flasks containing 100 mL of test solution under agitation in a climatic chamber. Flasks were incubated at a temperature of between 23.0 to 24.1°C with continious light in a range of 5 600 lux.
After 2', 48 and 72 hours, optical density (683 nm) were measured to determine cell concentrations in each replicate.
At the beginning of the test, test item solution was sampled (volume: 100 mL). After 24, 48 and 72 hours, test item solution was sampled (volume: 100 mL) in the seventh inoculated replicate of saturation concentration solution. These samples were quantified immediately after sampling according to the validated method.
The pH value for each condition was recorded at the beginning and the end of experimentation.
Reference substance (positive control):
yes
Remarks:
Quality control is performed 2 times per year with potassium dichromate to control breeding sensitivity

Results and discussion

Effect concentrations
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.03 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
See section "Any other information on results incl. tables" below
Results with reference substance (positive control):
Qualit control is performed 2 times per year with potassium dichromate to control breeding sensitivity

Any other information on results incl. tables

Measured data at 24, 48 and 72h (cells/mL)

 Test condition  16 May 2018 - 09 : 47 am (24h)  17 May 2018 - 09 : 51 am (48h)  18 May 2018 - 11 : 06 am (72h)
                Control  44205  150303  1213002
 75689  420439  1922795
 27914  178439  939086
 32989  162746  1229310
 26814  190556  931379
 42347  169010  1086743
                Saturation 62422   170002  1143046
64771   153933  831165
75193   166045  707991
68611   144782  993633
 92785  194450  907323
 86671  156088  786115

Growth rate and yield Inhibition per replicate

 Test condition  Growth rate (day-1)  Growth Inhibition (%)  Yield (cells/mL)  Yield inhibition (%)
                Control  1.8395   1207985   
 1.9940   1917778   
 1.7537   934069   
 1.8440   1224293   
1.7510     926362  
1.8027     1081726  
Saturation                1.8196 0.6   1138029 6.4 
 1.7128 6.4   826148 32.0 
 1.6591 9.4   702974 42.2 
 1.7727 3.2  988616  18.7 
 1.7422 4.8  902306  25.8 
 1.6941  7.5 781098  35.7 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Ecotoxicity test of butyl avocadate distillated on Pseudokirchneriella subcapitata fulfils the requirements of OECD Guideline No. 201 to determine ErC50-72h and EyC50 > 0.03 mg/L (saturation concentration).
Executive summary:

Objective

The scope of this study was, in compliance with the principles of Good Laboratory Practice (GLP), to determine the toxicity of butyl avocadate distillated on Pseudokirchneriella subcapitata (Algae) in a 72 -hour static test.

Method Summary

Saturation concentration in test medium was prepared and divided into 8 replicates. 7 replicates were inoculated in the range of 5 000 to 10 000 cells per mL. The 7th replicate was used as a colour control (not inoculated). 6 inoculated replicates were used to measure algal growth and 1 inoculated replicate was used for analytical monitoring.

A control (6 replicates) was tested at the same time with test medium inoculated in the range of 5 000 to 10 000 cells per mL.

Results

   Butyl avocadate distillated - based on measured concentration
 ErC50 -72h (Confidence limits 95%)  > 0.03 mg/L (N.A.)
 EyC50 -72h (Confidence limits 95%)  > 0.03 mg/L (N.A.)

ECX-72h: Effective Concentration corresponding to X% of inhibition in the 72- hours test

r: growth rate

y: growth yield

N.A.: Not Applicable

Conclusion

Ecotoxicity test of butyl avocadate distillated on Pseudokirchneriella subcapitata fulfils the requirements of OECD Guideline No. 201 to determine ErC50-72h and EyC50 > 0.03 mg/L (saturation concentration).