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EC number: 908-912-9 | CAS number: 1333-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- fish embryo acute toxicity (FET)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 5 March 2016
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 15088, 2007
- Deviations:
- yes
- Remarks:
- the exposure of the embryos was prolonged to 4 days
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-[(2-hydroxyphenyl)methyl]phenol; 2-[(4-hydroxyphenyl)methyl]phenol; 4-[(4-hydroxyphenyl)methyl]phenol
- EC Number:
- 908-912-9
- Cas Number:
- 1333-16-0
- Molecular formula:
- C39H36O6
- IUPAC Name:
- 2-[(2-hydroxyphenyl)methyl]phenol; 2-[(4-hydroxyphenyl)methyl]phenol; 4-[(4-hydroxyphenyl)methyl]phenol
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
Test solutions
- Vehicle:
- yes
- Remarks:
- ultrapure water
- Details on test solutions:
- Stock solutions were prepared by dissolving BPF (20 and 30 mg/L) in ultrapure water by mixing the solutions on a magnetic stirrer for at least 24 h. Chemicals were purchased from SigmaeAldrich (99% purity or higher). All chemicals used for toxicity testing were of analytical grade.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Adult zebrafish were bred in an aquarium (60 x 30 x 30 cm) containing 45 L of tap water at constant temperature (26 ± 1 °C) and 12 h light/12 h dark photoperiod. Fish were fed three times per day with commercially available dried fish food (TetraMin®, Tetra, Germany) and three times per week with young daphnids.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Salinity:
- not applicable as tap water used.
- Nominal and measured concentrations:
- 0.3, 0.6, 0.9, 1.2, 2.0, 4.0, 6.0, 8.0, 8.8, 10.0, 13.0, 16.0, 18.0, 20.0 mg/L
- Details on test conditions:
- In the acute toxicity test with zebrafish, 1 mL of tested concentrations BPF (0.3, 0.6, 0.9, 1.2, 2.0, 4.0, 6.0, 8.0, 8.8, 10.0, 13.0, 16.0, 18.0, 20.0 mg/L), and dilutionwater (control) were placed into
each hole of 24-well plates and then individual 3 hpf (3-h post fertilization) eggs were added in each hole; for each test concentration and a control ten fertilized eggs were exposed. In principle,
toxicity test with zebrafish embryos was performed according to ISO standard (ISO 15088, 2007), but the exposure of embryos was prolonged to 4 days. After 24 and 48 h of exposure, lethal (egg
coagulation, missing heartbeat, missing somites, missing tail detachment from the yolk sac) and sublethal (no eye and body pigmentation, missing blood flow, pericardial edema) malformations
were inspected using a stereoscopic microscope (Nikon SMZ 1000 with a DS-Fi1 digital camera) and a NIS-Elements Documentation 2.2 imaging software. Embryos were checked daily for mortality, developmental malformations and body length (a distance from the most anterior part of the head to the tip of the tail) after 96 h of exposure. At least three independent experiments were performed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: body and eye pigmentation
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- BPF induced malformations in development of zebrafish embryos. The lowest toxicity was observed in the case of BPF, since 20% of lethal effects and no effects on blood flow were observed at the highest tested concentration (20.0 mg/L). The most sensitive sublethal endpoint of BPF was body and eyes pigmentation of zebrafish embryos as the 48 h EC50 value was determined at 1.1 mg/L.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
LC/EC values of BPF for zebrafish embryos
BPF EC/LC (mg/L) 20 50
(95% CL)*80 Mortality (48 h) / / / Missing body pigmentation (48 h) 0.7 1.1
(0.92 - 1.3)1.8 Missing blood flow (48 h) / / / Pericardial edema (48 h) 9 10.7
(9.4 - 12.2)12.7 Hatching inhibition (72 h) 4 6.8
(5.5 - 8.5)11.7 / - EC/LC values were not determined as 20% of lethal effects and no effects on blood flow were observed at the highest tested concentration of BPF (20 mg/L).
* Corresponding 95% confidence limits.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The most sensitive sublethal endpoint of BPF was body and eye pigmentation as the 48 h EC50 value was determined at 1.1 mg/L. The EC50 for mortality is > 20.0 mg/L.
- Executive summary:
At test was carried out to determine the short term toxicity of bisphenol F on zebrafish. The method used was in accordance with ISO 10706, 2000.
The results showed that BPF induced malformations in development of zebrafish embryos. The lowest toxicity was observed in the case of BPF, since 20% of lethal effects and no effects on blood flow were observed at the highest tested concentration (20.0 mg/L) (EC50 >20 mg/L). The most sensitive sublethal endpoint of BPF was body and eyes pigmentation of zebrafish embryos as the 48 h EC50 value was determined at 1.1 mg/L.
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