Ethylene di(S-thioacetate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test and at day 3.For the determination of the stability of the test item under the test conditions, respectively the maintenance of the test item concentrations during the test period, samples were taken in duplicate out of all test media and the control at day1 and at the end of the test (96 hours) from the approximate centre of the aquaria.

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Size and Weight: Juveniles;The mean body length of the fish* in the test was 4.89 cm ± 0.4 cm (Mean ± SD), the mean body wet weight 1.20 g ± 0.3 g (Mean ± SD).* 10 fish from the test fish batch were measured before the start of the testSex: Male and femaleOrigin: The test fish were obtained from Forellenzuchtbetrieb Störk, 88348 Bad Saulgau, Germany Holding Conditions: All fish were obtained and held in the laboratory for at least 12 days before the start of the test. They were held in water of the quality to be used in the test for at least seven days immediately before testing under the following conditions:Light: 16 hours photoperiod dailyTemperature: 13 - 17 °C Oxygen concentration: at least 80 % of the air saturation valueFeeding: three times per week or daily until 24 hours before the test was started During the last 7 days prior to the start of the test no fish (0 %) died in the test fish batch. Therefore the mortalities in the fish batch were below 5 % and the fish batch was accepted.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

pH:

7.5 to 7.9

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.508 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Result will be added when study is completed.

Sublethal observations / clinical signs:

Table 1. Observed Mortality of unfed Rainbow Trout (Oncorhynchus mykiss) exposed to GDMA for 96 hours

Nominal Concentration	Mortality [Number of Dead]
mg test item/L	0 h	24 h	48 h	72 h	96 h
Control	0	0	0	0	0
0.11	0	0	0	0	0
0.24	0	0	0	0	0
0.54	0	0	0	2	4
1.2	0	0	2	7	7
2.6	0	7	7	7	7
LC50 [mg test item/L]	-	-	-	-	0.508
95% CI.	-	-	-	-	0.376 - 0.687

- not relevant

CI.: Confidence interval
Values refer to nominal test concentrations.

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item GDMA to Oncorhynchus mykiss was
assessed in a semi-static concentration-response test. The 96-hour
EC50 was determined to be 0.508 mg test item/L.
The initial concentrations and the maintenance of the exposure
concentrations during the test were examined in the analytical part.
All reported results refer to nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.58 mg/L