Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
equivalent or similar to
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Qualifier:
according to
Guideline:
ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Duration of test (contact time):
29 d
Initial conc.:
51 mg/L
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
acetic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
13
Sampling time:
29 d
Details on results:
Points of degradation plot (test substance, average of 2 tests):
1 % degradation after 2 d
2 % degradation after 5 d
3 % degradation after 7 d
3 % degradation after 9 d
5 % degradation after 14 d
6 % degradation after 19 d
10 % degradation after 23 d
12 % degradation after 27 d
13 % degradation after 29 d
Results with reference substance:
Points of degradation plot (reference substance):
3 % degradation after 2 d
37 % degradation after 5 d
57 % degradation after 7 d
66 % degradation after 9 d
71 % degradation after 14 d

The test substance was not readily biodegradable under the conditions of the modified Sturm test presently performed.

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable, not fulfilling specific criteria
Executive summary:

Determination of ready biodegradability: CO2 evolution test with Everzol Orange ED-G Crude. The concentration of Everzol Orange ED-G was 51 mg/l. The test substance was added to duplicate test bottles and the toxicity control.

The relative degradation values calculated from the measurements performed during the test revealed 19 and 8% degradation of Everzol Orange ED-G Crude, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was no met. In the toxicity control, Everzol Orange ED-G crude was found not to inhibit microbial activity.

In conclusion, Everzol Orange ED-G Crude is not readily biodegradable.

Description of key information

The test substance was not readily biodegradable under the test conditions.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, not fulfilling specific criteria

Additional information

Determination of ready biodegradability: CO2 evolution test with Everzol Orange ED-G Crude. The concentration of Everzol Orange ED-G was 51 mg/l. The test substance was added to duplicate test bottles and the toxicity control.

The relative degradation values calculated from the measurements performed during the test revealed 19 and 8% degradation of Everzol Orange ED-G Crude, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was no met. In the toxicity control, Everzol Orange ED-G Crude was found not to inhibit microbial activity.

In conclusion, Everzol Orange ED-G Crude is not readily biodegradable.