Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 605-620-0 | CAS number: 171869-89-9
Skin sensitization (OECD 429), mouse: sensitising
Skin sensitiztion (OECD 406, Buehler), guinea pigs: sensitising
Table 1: Summary of LLNA result
Concentration (% in (v/v) DFM
mean dpm ± standard deviation
558.6 ± 308.7
3128.6 ± 2613.3
6086.8 ± 3463.8
5263.2 ± 1935.4
6467.6 ± 2983.5
Two reliable in vivo skin sensitisation studies are available with the test substance (CAS 171869-89-9).
The key study is a LLNA test in mice according OECD 429 and in compliance with GLP (Dow Corning, 2005). The dose levels were selected based on an irritancy screen and were set to 20, 40 and 80%. The stimulation indices (SI) obtained with the test material at concentrations of 20, 40 and 80% in DMF (v/v) were greater than 3 in each case (5.6, 10.9 and 9.4%, respectively) with statistical evidence of a dose-response (Dow Corning, 2005). The stimulation indices were used to determine an EC3 value of 7%. Proper conduct of the study was verified by positive response (SI = 11.6) using α-hexylcinnamaldehyde as a positive control. Based on these results, the test substance is considered positive for contact sensitisation.
In a supporting study according to OECD 406 and in compliance with GLP the skin sensitising potential of the registration substance was assessed using guinea pig (Dow Corning, 2001). The method used was a modification of that described by Buehler, E.V. (1965), Delayed contact hypersensitivity in the guinea-pig. Based on the results of a preliminary study the test substance was applied as neat substance in the three induction applications as well as in the challenge application. In this study the test substance produced clear evidence of skin sensitisation in six animals of the twenty test animals (= 30%). Eight animals gave inconclusive responses and the remaining six of the twenty animals gave negative responses. As more than 15% of the animals gave positive responses, the test substance is considered to have the potential to cause skin sensitisation. All animals in the positive control group produced evidence of skin sensitisation following treatment with hexylcinnamaldehyde.
The available data on skin sensitisation meet the criteria for classification according to Regulation (EC) 1272/2008, and is therefore classified as Skin Sensitising Cat. 1B (H317).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again