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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Soybean meal
EC Number:
269-648-1
EC Name:
Soybean meal
Cas Number:
68308-36-1
IUPAC Name:
Soybean meal

Sampling and analysis

Analytical monitoring:
yes
Remarks:
TOC

Test solutions

Vehicle:
yes
Remarks:
water

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
other: deionised water
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.5 mmol/L (= 250 mg/L) as CaCO3
Test temperature:
Water temperature: 19.2 to 21.0 °C
pH:
7.5 to 8.5
Dissolved oxygen:
8.4 to 9.7 mg/L
Nominal and measured concentrations:
Nominal concentrations: 100, 50, 25, 12.5 and 6.25 mg test item/L.
All reported results refer to nominal values since the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.
Details on test conditions:
Photoperiod: 16 h light - 8 h dark
Light intensity: 580 to 800 lux;
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
48h EC50 = 1.02 mg/L
48h NOEC = 0.25 mg/L
48h LOEC = 0.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The toxic effect of the test item Soya flour to Daphnia magna was assessed in a semi-static concentration-response test.
The 48-hour NOEC was determined to be ≥ 100 mg test item/L. The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was calculated to be > 100 mg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part.
All reported results refer to nominal values since the measured concentrations of the carbon content of the test item were not quantifiable because of the poor solubility of the test item.

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