2-ethyl-2-oxazoline

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethyl-2-oxazoline

EC Number:

233-912-4

EC Name:

2-ethyl-2-oxazoline

Cas Number:

10431-98-8

Molecular formula:

C5H9NO

IUPAC Name:

2-ethyl-4,5-dihydro-1,3-oxazole

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

The animals were placed in individual holding cages with free access to food and water.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

Acute percutaneous absorption toxicity was evaluated by application of 500 mg/kg of the active material as a 10% aqueous solution to the intact skin of 2 male and 2 female rabbits and 500 or 252 mg/kg to abraded skin on similar groups of rabbits. Twenty-four hours prior to application, the entire trunk of each rabbit was clipped free of hair with electric clippers. The material was applied under a heavy gauge plastic cuff which was held in place by rubber bands. The plastic cuff was covered with a cloth bandage taped securely to the marginal hair. After 24 hours the cuffs were removed, and the skin was washed with soap and water, rinsed thoroughly, and dried with a cloth towel.

Doses:

500 mg/kg (as a 10% aqueous solution) to intact skin
500 or 252 mg/kg to abraded skin

No. of animals per sex per dose:

2 males and 2 females per dose

Control animals:

no

Details on study design:

The topical response at the site of application was evaluated after removal of the plastic cuff. The animals were observed frequently during exposure and for the following two weeks for signs of toxicity. Body weights were recorded before and after the 24-hour exposure period and at 1 and 2 weeks post-treatment, or until pre-treatment weight was regained.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 500 mg/kg bw

Based on:

test mat.

Mortality:

The 2 female rabbits from the 500 mg/kg group with abraded skin died 17 and 18 days after treatment.

Clinical signs:

other: The animals with intact skin exhibited slight to moderate redness and slight to severe swelling when the cuffs were removed; the animals with abraded skin, exhibited very slight redness and swelling (252 mg/kg group); and slight to severe redness with sli

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

No deaths were reported following unabraded dermal exposure of 2 male and 2 female rabbits to 500 mg/kg bw active material presented as a 10% aqueous solution of ETOX.