Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian somatic and germ cell study: gene mutation
Remarks:
genotoxic effects in somatic and meiotic tissue
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1989

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Type of assay:
other: genotoxic effects in somatic and meiotic tissue

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylfuran
EC Number:
208-594-5
EC Name:
2-methylfuran
Cas Number:
534-22-5
Molecular formula:
C5H6O
IUPAC Name:
2-methylfuran
Specific details on test material used for the study:
not specified

Test animals

Species:
mouse
Strain:
Swiss
Remarks:
albino
Details on species / strain selection:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
test animals:
age: 8 to 10 weeks

Administration / exposure

Route of administration:
oral: feed
Vehicle:
not specified
Details on exposure:
somatic test system: feeding: 24 h interval for five days
meiotic test system: 24 h feeding
Duration of treatment / exposure:
somatic test system: 5 days
meiotic test system: 1 day
Frequency of treatment:
24 h
Post exposure period:
somatic test system: scoring after 24, 48 and 72 hours after oral feeding
meiotic test system: 1 to 5 weeks after exposure
Doses / concentrationsopen allclose all
Dose / conc.:
1 000 ppm
Dose / conc.:
2 000 ppm
Dose / conc.:
4 000 ppm
No. of animals per sex per dose:
not specified
Control animals:
not specified
Positive control(s):
not specified

Examinations

Tissues and cell types examined:
somatic test sytem: bone marrow cells (synthesis of spindle proteins, cell division)
meiotic test system: sperm cells (spermatogenetic cycle, sperm abnormalities)
Details of tissue and slide preparation:
not specified
Evaluation criteria:
not specified
Statistics:
not specified

Results and discussion

Applicant's summary and conclusion

Conclusions:
Under the conditions of the test, the test substance did not induce chromosome mutations in bone marrow cells, inhibit synthesis of spindle proteins or retardation of cell division. In a meiotic test system, the test substance did not induce genotoxic effects or causing sperm head abnormalities. In conclusion the test substance did not cause any genotoxic hazards to the mammalian test system.
Executive summary:

In this study 2-methylfuran was evaluated by a cytogenetic assay employing somatic and meiotic tissues on 8 to 10 week old Swiss albino mice as test system.

In the somatic test system chromosome mutations were scored from bone marrow cells, 24, 48 and 72 h after oral feeding at 1000, 2000 and 4000 ppm at 24 h intervals for five days. The test substance did not induce chromosome mutations in bone marrow cells, inhibit synthesis of spindle proteins or retardation of cell division.

In a meiotic test system, the test was carried out for 24 h and scored from 1 to 5 weeks at weekly intervals to cover one spermatogenetic cycle. The test substance did not induce genotoxic effects or causing sperm head abnormalities.

In conclusion, under the conditions of the test the test substance did not cause any genotoxic hazards to the mammalian test system.

The assay was not performed according to an OECD TG nor in compliance to GLP.