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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1993-05-04 to 1993-06-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
EG 84/449, 1989
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Part 1, 1984
Deviations:
no
Qualifier:
according to
Guideline:
other: DIN 38412 Part 1
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
A stock solution was prepared with concentration of 1 g/L in deionised water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Waterflea
- Clone: 5
- Strain: Straus
- Source: in-house culture.
The culture is kept in M4 Medium in 1 L beakers. Every 2-3 days the medium is changed and the young daphnids are separated.
- Feeding of the culture
- Food type: Scenedesmus subspicatus
- Amount: calculated in order for the animals to consume the whole amount
- Frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 °C
pH:
7.8 - 8.3
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0, 0.8, 1.5, 3, 6, 12, 25, 50, 100 mg/L
No measured concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: round bottom glasses
- Material, size, headspace, fill volume: glass, 10 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding during the test: no

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, prepared according to OECD 202
- Composition of test medium:
CaCl2x2H2O - 294 mg/L
MgSO4x7H2O - 123 mg/L
NaHCO3 - 63 mg/L
KCl - 5.5 mg/L
- Na/K ratio: 10:1
- Ca/mg ratio: 2.5 mmol; 4:1
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous darkness
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
31.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CI: 22.9 - 43.9 mg/L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
19.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CI: 14.1 - 27.7 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
After 48 h the highest concentration at which no immobilisation (<= 10 %) was observed, was determined to be 3 mg/L.
After 48 h the highest concentration at which 100 % immobilisation was observed, was determined to be 100 mg/L.
All biological effects are based on the nominal concentrations.
Results with reference substance (positive control):
Potassium dichromate was tested as reference substance. After 24 h exposure at concentration level of 0.9 mg/L 30 % immobilisation was observed. 100 % immobilisation was observed at 1.9 mg/L.

Table 1. Individual results on mobility after 24 and 48 h

Concentration (mg/L)

24 h

48 h

Animals

%

Animals

%

Mobile

Immobile

Immobile

Mobile

Immobile

Immobile

Control

19

1

5

19

1

5

0.8#

19

0

0

18

1

5

1.5*

21

0

0

21

0

0

3.0

20

0

0

20

0

0

6.0

19

0

0

17

2

11

12.0

16

4

20

11

9

45

25.0

15

5

25

12

8

40

50.0

6

14

70

3

17

85

100.0

0

20

100

0

20

100

# 21 daphnids were counted at the beginning of the test.

* 19 daphnids were counted at the beginning of the test.

Table 2. EC50 values calculated after 24 and 48 h exposure

Time (h)

EC50 (mg/L)

95 % confident intervals (mg/L)

24

31.7

22.9 – 43.9

48

19.7

14.1 – 27.7

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of the test item on Daphnia magna was determined after 48 h exposure to be 19.7 mg/L. The NOEC was determined to be 3 mg/L.
Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and DIN 38412 Part 1. The study was conducted in a static system over a period of 48 hours with a concentration series of 0.8, 1.5, 3, 6, 12, 25, 50 and 100 mg/L. 20 daphnids per test concentration and control divided in 5 replicates with 4 animals each were tested. The test item concentration was not determined analytically, therefore, the biological endpoints were based on the nominal concentration. After 48 h exposure significant effects on the daphnids were observed. The NOEC was determined to be 3 mg/L. The concentration at which 100 % immobilization was observed was determined to be 100 mg/L. The EC50 after 48 h exposure was determined to be 19.7 mg/L. Significant effects of the test item on Daphnia magna were observed under the test conditions.

Description of key information

The EC50 of the test item on Daphnia magna was determined after 48 h exposure to be 19.7 mg/L. The NOEC was determined to be 3 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
19.7 mg/L

Additional information

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and DIN 38412 Part 1. The study was conducted in a static system over a period of 48 hours with a concentration series of 0.8, 1.5, 3, 6, 12, 25, 50 and 100 mg/L. 20 daphnids per test concentration and control divided in 5 replicates with 4 animals each were tested. The test item concentration was not determined analytically, therefore, the biological endpoints were based on the nominal concentration. After 48 h exposure significant effects on the daphnids were observed. The NOEC was determined to be 3 mg/L. The concentration at which 100 % immobilization was observed was determined to be 100 mg/L. The EC50 after 48 h exposure was determined to be 19.7 mg/L. Significant effects of the test item on Daphnia magna were observed under the test conditions.