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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
purity of test substance; acclimation period, details on test animals and housing/feeding conditions not reported

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
purity of test substance; acclimation period, details on test animals and housing/feeding conditions not reported
Principles of method if other than guideline:
Not applicable
GLP compliance:
no
Remarks:
pre-GLP
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4,6-trinitrobenzene-1,3,5-triamine
EC Number:
221-297-5
EC Name:
2,4,6-trinitrobenzene-1,3,5-triamine
Cas Number:
3058-38-6
Molecular formula:
C6H6N6O6
IUPAC Name:
trinitrobenzene-1,3,5-triamine
Test material form:
not specified
Details on test material:
- Name of test material as cited in the report: TATB ; 1,3,5-triamino-2,4,6-trinitrobenzene
- Lot/batch No.of test material: B-318

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Details on oral exposure:
Test substance was administered to fasted rats as a suspension in corn oil.
Doses:
5000 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 2 weeks
- Frequency of observations and weighing: After treatment, all animals were observed daily for physiological and behavioural responses. Body weights were recorded initially and weekly thereafter.
- Necropsy of survivors performed: Yes; at the end of the 2-week observation period, all surviving animals were sacrificed and examined for evidence of gross pathological changes.
Statistics:
None

Results and discussion

Preliminary study:
Range-finding study results not reported.
Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality was observed.
Clinical signs:
other: All animals appeared to be normal throughout dosing and during the post-treatment observation period.
Gross pathology:
No abnormalities were noted at necropsy of animals that were killed at the end of the study.
Other findings:
None

Any other information on results incl. tables

None

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Under the test conditions, the oral LD50 of the test substance is higher than 5000 mg/kg bw therefore it is not classified for acute oral toxicity according to the CLP Regulation EC No. (1272/2008) and to the GHS.
Executive summary:

In an acute oral toxicity study, Sprague-Dawley rats (10 animals/sex/dose) were given a single oral (gavage) dose of test substance at 5000 mg/kg bw. Animals were then observed for mortality, clinical signs and bodyweights for 2 -weeks and at the end of the study the surviving animals were sacrificed for macroscopic examination.

No mortalities occurred and no signs of systemic toxicity were observed during the 14-day observation period. All animals showed body weight gain during the study period. No abnormalities were noted at necropsy of animals that were killed at the end of the study.

Under the test conditions, the oral LD50 of the test substance is higher than 5000 mg/kg bw therefore it is not classified for acute oral toxicity according to the CLP Regulation EC No. (1272/2008) and to the GHS.