Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

IUPAC Name:

Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

Test material form:

liquid

Specific details on test material used for the study:

Batch number : 08E20

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Earthworms were obtained from Blades Biological, Cowden, Edenbridge, Kent, UK and maintained in a 20 L platic tray at a temperature of approximately 21°C on a substrate consisting of 50% by volume peat and 50% by volume animal manure.

The test organisms were with clitellum and were transferred into artificial soil approximately 24 hours prior to the start of the test.

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

21-23°C

pH:

6.0 ± 0.5

Moisture:

24-30%

Details on test conditions:

exposure in 1 litre glass beakers
10 earthworms per replicate
4 replicates for control
6 replicates for 1000 mg/kg treatment

artificial soil:
quartz sand: 70%
kaolinite clay: 20%
spaghnum moss peat: 10%

Nominal and measured concentrations:

nominal conentration: 1000 mg/kg soil.

Reference substance (positive control):

yes

Remarks:

positive control conducted every 6 months using chloroacetamide as reference material

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

weight

Details on results:

There were no significant mortalities or sub-lethal effects of exposure in 60 earthworms exposed to a test concentration of 1000 mg/kg for a period of 14 days.

There were three worms unaccounted for after Day 14 in the control and four worms unaccounted for after Day 14 in the 1000 mg/kg test concentration. This was considered not to affect the outcome or validity of the test as the overall mean mortality value was less than 10% in both the controls and the 1000 mg/kg test concentration.

It was considered unnecessary and unrealistic to test at concentrations in excess of of 1000 mg/kg.

The results of the definitive test showed the highest test concentration resulting in 0% mortality to be greater than or equal to 1000 mg/kg and the lowest test concentration resulting in 100% mortality to be greater than 1000 mg/kg.

There were no observed sub-lethal effects of exposure of Eisena foetida to the test material.

Results with reference substance (positive control):

The NOEC was 10 mg/kg. The results from the positive control with chloroacetamide are within the normal range for this material of 10 to 50 mg/kg.

Reported statistics and error estimates:

There were no significant differences (p>0.05) between the control and 1000 mg/kg test groeps in terms of weight on Days 0 and 14.

Applicant's summary and conclusion

Conclusions:

The acute toxicity of he test material, to the earthworm Eisenia foetida, in an artificial soil test has been investigated and gave a 14-Day LC50 value of greater than 1000 mg/kg. Correspondingly the NOEC was 1000 mg/kg.