Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-230-0
CAS number: -
Several studies on mouse were available on
butane-1,4-diylbis(oxy-2-hydroxypropane-3,1-diyl) bisacrylate to
evaluate the skin sensitization potential. Based on the data,
butane-1,4-diylbis(oxy-2-hydroxypropane-3,1-diyl) bisacrylate is
considered as a moderate skin sensitizer.
No signs of systemic toxicity were observed. The body weight increased
Moderate redness was noted between the ears in animals treated with 10%
and 25% of the test substance.
A local lymph node assay in the mouse was performed on
butane-1,4-diylbis(oxy-2-hydroxypropane-3,1-diyl) bisacrylate to assess
the skin sensitization potential of the substance in the CBA/Ca strain
mouse following topical application to the dorsal surface of the ear.
The study was performed according to the OECD guideline 429 (2002).
Following a preliminary screening test, three groups, each of four
animals, were treated with 50 µl (25 µl per ear) of the test material as
a solution in acetone/olive oil 4:1 at concentrations of 5%, 10% and
25%. A further group of 4 animals was treated with acetone/olive oil 4:1
The stimulation index expressed as the mean incorporation for each
treatment group divided by the mean radioactive incorporation of the
vehicle control group are of 4.28, 4.14 and 7.32 respectively. All the
SI were higher than 3, indicating positive results. In conclusion, the
test material was considered to be a sensitizer under the conditions of
Key study (2005) :
Supporting studies (2002):
In the first LLNA experiment, 1%, 3%, and 10% preparations of the test
substance in propylene glycol were administered to the ears of 6 female
CBA/Ca mice per group. A positive response (cell count index of 2.16 and
lymph node weight increase of 78%) was observed in the 10% group only,
but at the same time the ear weight was increased by 31%, which is
considered a sign for excessive irritation. Therefore it was impossible
to determine, if the effects were due to the sensitzing or irritating
properties of the test substance.
To clarifiy the results, the second study was performed using a
preparation of 6.5% in propylene glycol according to two different
treatment regiments: either the mice were treated with 25µl on both ear
on three consecutive days as in the previous experiment (induction phase
group), or they were treated with 25µl on the flank for three
consecutive days 14 days prior to exposure to 25µl on the ears on three
consecutive days (challenge phase group). In both cases no increase in
ear weight and thus no sign for local irritation was observed. The
induction phase group only showed a slight and not significant increase
in lymph node weights and cell count, while the challenge phase group
exhibited an increase in lymph node weight of 80% and a cell count index
of 2.06 when compared to control.
Based on the available data,
butane-1,4-diylbis(oxy-2-hydroxypropane-3,1-diyl) bisacrylate should be
classified as moderate skin sensitizer (Skin sens.1B, H317).
Justification: Excessive irritation was observed in all studies at and
above a concentration of 10%, while at concentrations of 5% (2005) and
6.5% (2002) sensitization was observed without irritation. Since no
sensitization occurred when using a 1% or 3% solution (2002), the
threshold for sensitization is above 3% and below 5%.
There are no data available to classify the substance
as a sensitizer of the respiratory tract.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again