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This aquatic toxicity assessment was based on the following data:

The short-term toxicity of the test substance to fish was investigated in a GLP guideline study according to OECD 203 (BASF SE 17F0446/01X048, 2013). The LC50 (96h) on Danio rerio was determined to be 66.8 mg/L, based on analytically verified nominal concentrations.

The toxicity of the test substance to aquatic invertebrates was investigated in a GLP guideline study according to OECD 202 (BASF AG 99/0247/50/1, 1999). The EC50 (48h) on Daphnia magna was determined to exceed the only tested, analytically verified nominal test concentration of 100 mg/L (limit test).

The toxicity of the test substance to aquatic algae was investigated in a GLP guideline study according to OECD 201 (BASF SE 60E0446/01X049, 2013). The ErC50 (72h) on Pseudokirchneriella subcapitata was determined to be 61.6 mg/L, based on analytically verified nominal concentrations.

The ErC10 (72h) was determined to be 22.5 mg/L, based on analytically verified nominal concentrations.

The toxicity of the test substance to microorganisms was investigated by the conduction of an inhibition control in the framework of a ready biodegradability study (OECD 301F, BASF AG 486504, 1986). The domestic, activated sludge used as inoculum showed no significant inhibition of its degradation potential after a test duration of 28 days at a test substance concentration of 100 mg/L. This result is supported by the outcome of a guideline study similar to OECD 209 for which only a summary is available. In this study, an EC50 (30 min) > 100 mg/L was determined (BASF AG 1363, 1986). Based on these data, the inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.