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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: Propanenitrile, 2-[bis(cyanomethyl)amino]
- Molecular formula: C7H8N4
- Molecular weight: 148.17
- Impurities: < 0.1 g/100 g water, 0.08 g/100 g nitrilotriacetonitrile, free hydrocyanic acid 14.5 mg/kg
- Purity: 99.9g/100g %

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Municipal activated sludge from the wastewater treatment plant of Mannheim/Baden Württemberg (Germany). The inoculum was collected on 19 June 2006 from the aeration tank of the plant and aerated in the Iaboratory until use. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size of about 1 mm. After settling the supernatant was discarded and the sludge suspension was filled up with tap water. This suspension was pre-aerated for about 24 hours at room temperature. The sludge of the suspension was adjusted to a concentration of 6.0 gIL dry weight and then added to the test vesseis to obtain a sludge concentration of 30 mg/L dry substance. At last the activated sludge suspension in the test vesseis was pre-aerated once again for about 24 hours at a temperature of 22±2 °C.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
30 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
10 - 20
Sampling time:
28 d
Details on results:
Degradation in the inhibition control after 14 days: 30 %

BOD5 / COD results

Results with reference substance:
Degradation of reference substance after 14 days: 82 %

Applicant's summary and conclusion

Interpretation of results:
other: poorly biodegradable