Pyridinium, 1-[4-[[4-[2-[5-(acetylamino)-2-methoxy-4-[2-(3,6,8-trisulfo-2-naphthalenyl)diazenyl]phenyl]diazenyl]-8-sulfo-1-naphthalenyl]amino]-6-amino-1,3,5-triazin-2-yl]-3-carboxy-, inner salt, sodium salt (1:?)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pyridinium, 1-[4-[[4-[2-[5-(acetylamino)-2-methoxy-4-[2-(3,6,8-trisulfo-2-naphthalenyl)diazenyl]phenyl]diazenyl]-8-sulfo-1-naphthalenyl]amino]-6-amino-1,3,5-triazin-2-yl]-3-carboxy-, inner salt, sodium salt (1:?)

EC Number:

691-442-9

Cas Number:

1316184-44-7

Molecular formula:

C38H29N11O16S4 . x Na x=1~4

IUPAC Name:

Pyridinium, 1-[4-[[4-[2-[5-(acetylamino)-2-methoxy-4-[2-(3,6,8-trisulfo-2-naphthalenyl)diazenyl]phenyl]diazenyl]-8-sulfo-1-naphthalenyl]amino]-6-amino-1,3,5-triazin-2-yl]-3-carboxy-, inner salt, sodium salt (1:?)

Test solutions

Details on test solutions:

The standard test procedures required generation of test solutions, which should contain completely dissolved test substance concentrations or stable and homogeneous mixtures or dispersions. The testing of concentrations that disturb the test system should be prevented (e.g. film of the test substance on the water surface.)
Preparation of test solutions started with a concentration of 100 mg/l. No other treatment than vigorous shaking was needed to completely dissolve the test substance in the medium. The lower test concentration for the range-finding test were prepared by subsequent dilutions of the highest concentration in test medium. The final test soutions were all clear and ranged from colourless to orange. No correction was made for purity/composition of the test substance.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, 1820
- Source: In-house laboratory culture with a known history.
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: After 7 days of cultivation half of the medium twice a week.
- Feeding during test
- Food type: a suspension of fresh water algae.
- Frequency: daily

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

At 24 hours and at 48 hours.

Test conditions

Hardness:

180 mg/l expressed as CaCO3 and the pH: 7.7 ± 0.3.

Test temperature:

18~22℃

pH:

6.0-8.5

Dissolved oxygen:

>= 3 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Any other information on results incl. tables

Table 2 Acute immobilisation of daphnids after 24 and 48 hours in the limit test

Nominal concentration Everzol SB45 (mg/l)	Vessel number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	Total %	number	Total %
control	A	5	0	0	1	5
	B	5	0		0(1)
	C	5	0(1)		0
	D	5	0		0(1)
100	A	5	0	0	0	0
	B	5	0		0
	C	5	0		0
	D	5	0		0
( )between brackets : number of daphnia observed trapped at the surface of the test solutions. These organisms were reimmersed into the respective solutions before recording of mobility.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study Everzol SB45 did not induce acute immobilisation of Daphnia magna at an analytically confirmed nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 for immobility was beyond the range tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/l.