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Physical & Chemical properties

Water solubility

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Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Principles of method if other than guideline:
A purified, lyophilized Subtilisin Carlsberg (Sigma) was dissolved at 25°C in dest. water up to the dissolving limit (formation of sediment at the bottom of the test tube). An appropriate amount of liquid was transferred to a weighted test tube, frozen and lyophilized. The remaining protein content was measured gravimetrically.
GLP compliance:
no
Type of method:
other: gravimetrically measurement
Water solubility:
> 100 g/L
Temp.:
25 °C
pH:
7.8
Remarks on result:
other: Note: Data was published in HERA 2007 as “> 1 kg/L”, but has to be corrected to “> 100 g/L”.
Details on results:
For this sample a water solubility of 280 to 290 g/L was found.
Conclusions:
Interpretation of results: very soluble (> 10000 mg/L)
The water solubility of the test substance is above 100 g/L thus the test substance is very soluble in water.
Endpoint:
water solubility
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
refer to analogue justification provided in IUCLID section 13
Reason / purpose:
read-across source
GLP compliance:
no
Water solubility:
> 100 g/L
Temp.:
25 °C
pH:
7.8
Remarks on result:
other: Source: CAS 9014-01-1, HERA, 2007, RL1
Conclusions:
Interpretation of results: very soluble (> 10000 mg/L)
The water solubility of Thermomycolin is above 100 g/L and therefore it is very soluble in water.
Executive summary:

Result of water solubility for source substance, Subtilisin, CAS 9014 -01 -1: Very soluble (> 10000 mg/L).

Thermomycolin shares the same structural features as Subtilisin as explained in the Analogue Approach Justification document provided in Section 13. Thus, based on the read-across from the structural analogue substance Subtilisin (CAS 9014-01-0), the target substance Thermomycolin (CAS 52233-31-5) is considered to possess the water solubility as the source.

Description of key information

The water solubility of the test substance is above 100 g/L and thus the substance is very soluble in water.

Key value for chemical safety assessment

Additional information