Registration Dossier
Registration Dossier
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EC number: 822-334-3 | CAS number: 52233-31-5
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Principles of method if other than guideline:
- A purified, lyophilized Subtilisin Carlsberg (Sigma) was dissolved at 25°C in dest. water up to the dissolving limit (formation of sediment at the bottom of the test tube). An appropriate amount of liquid was transferred to a weighted test tube, frozen and lyophilized. The remaining protein content was measured gravimetrically.
- GLP compliance:
- no
- Type of method:
- other: gravimetrically measurement
- Water solubility:
- > 100 g/L
- Temp.:
- 25 °C
- pH:
- 7.8
- Remarks on result:
- other: Note: Data was published in HERA 2007 as “> 1 kg/L”, but has to be corrected to “> 100 g/L”.
- Details on results:
- For this sample a water solubility of 280 to 290 g/L was found.
- Conclusions:
- Interpretation of results: very soluble (> 10000 mg/L)
The water solubility of the test substance is above 100 g/L thus the test substance is very soluble in water. - Endpoint:
- water solubility
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- refer to analogue justification provided in IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- no
- Water solubility:
- > 100 g/L
- Temp.:
- 25 °C
- pH:
- 7.8
- Remarks on result:
- other: Source: CAS 9014-01-1, HERA, 2007, RL1
- Conclusions:
- Interpretation of results: very soluble (> 10000 mg/L)
The water solubility of Thermomycolin is above 100 g/L and therefore it is very soluble in water. - Executive summary:
Result of water solubility for source substance, Subtilisin, CAS 9014 -01 -1: Very soluble (> 10000 mg/L).
Thermomycolin shares the same structural features as Subtilisin as explained in the Analogue Approach Justification document provided in Section 13. Thus, based on the read-across from the structural analogue substance Subtilisin (CAS 9014-01-0), the target substance Thermomycolin (CAS 52233-31-5) is considered to possess the water solubility as the source.
Referenceopen allclose all
Description of key information
The water solubility of the test substance is above 100 g/L and thus the substance is very soluble in water.
Key value for chemical safety assessment
Additional information
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