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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Remarks:
estimated
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
Method in accordance to REACH guidance on QSARs: Chapter R.6. QSARs and grouping of chemicals
Qualifier:
according to
Guideline:
other: REACH guidance on QSARs: Chapter R.6. QSARs and grouping of chemicals
Deviations:
no
Principles of method if other than guideline:
EPI SUITE (BIOWIN v4.11)
Specific details on test material used for the study:
α - tocopheryl hydrogen succinate
CAS: 4345-03-3
SMILES: CC1=C2C(=C(C(=C1C)OC(=O)CCC(=O)O)C)CCC(O2)(C)CCCC(C)CCCC(C)CCCC(C)C
MW: 530.78
Oxygen conditions:
other: not applicable
Inoculum or test system:
other: not applicable
Key result
Parameter:
half-life in days (QSAR/QSPR)
Remarks on result:
not readily biodegradable based on QSAR/QSPR prediction
Details on results:
 Probability of Rapid Biodegradation (BIOWIN v4.11):
  Biowin1 (Linear Model): 0.9084
  Biowin2 (Non-Linear Model): 0.9538
 Expert Survey Biodegradation Results:
  Biowin3 (Ultimate Survey Model): 1.9614 (months)
  Biowin4 (Primary Survey Model) : 3.3460 (days-weeks)
 MITI Biodegradation Probability:
  Biowin5 (MITI Linear Model):  0.4028
  Biowin6 (MITI Non-Linear Model):  0.1257
 Anaerobic Biodegradation Probability:
  Biowin7 (Anaerobic Linear Model): -0.9281
 Ready Biodegradability Prediction:  NO

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey model) result is "weeks" or faster (i.e. "days", "days to weeks", or "weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then the prediction is YES (readily biodegradable). If this condition is not satisfied, the prediction is NO (not readily biodegradable). This method  is based on application of Bayesian analysis to ready biodegradation data. Biowin5 and 6 also predict ready biodegradability, but for degradation in the OECD301C test only; using data from the Chemicals Evaluation and Research Institute Japan (CERIJ) database.

Validity criteria fulfilled:
not applicable
Interpretation of results:
not readily biodegradable
Conclusions:
Under the study conditions, the test substance is considered not readily biodegradable.
Executive summary:

The ready biodegradability of the substance was predicted through EPI SUITE (BIOWIN v4.11). The substance was not found to be readily biodegradable based on overall results from several BIOWIN predictions (US EPA, 2012).

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: RA study
Remarks:
non-GLP; no details on test substance characteristics
Justification for type of information:
Refer to the section 13 for details on the read across justification.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, adapted
Duration of test (contact time):
39 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Key result
Parameter:
% degradation (O2 consumption)
Value:
> 30 - < 40
Sampling time:
39 d
Details on results:
Kinetic of test substance (in %):
< 10 after 19 day(s)
= 0.1 ... 11.2 after 20 day(s)
= 8.9 ... 23.8 after 25 day(s)
= 21.7 ... 31 after 28 day(s)
= 37.8 ... 41.4 after 39 day(s)
Results with reference substance:
Kinetic of control substance (in %):
< 10 after 5 day(s)
= 62.7 after 14 day(s)
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Based on the results from the read across study, the degree of biodegradation reached 30 - 40 % after 39 days. Therefore the test substance is considered to be not readily biodegradable.
Executive summary:

A study was conducted to determine the ready biodegradability of the read-across substance according to OECD Guideline 301 C. This study was performed in order to evaluate aerobic elimination and degradation potential of the test substance throughout a 39-day period in a test for ready biodegradability, using a nominal test substance concentration of 100 mg/mL in an activated industrial sludge, (adapted model). The degradation was followed by determination of the oxygen consumption. Measurements were taken at the following days: 19, 20, 25, 28 and 39. Based on the results of the read across study, the degree of biodegradation reached 30 - 40 % after 39 d, therefore the test substance is considered to be not readily biodegradable (BASF, 1988).

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

Study 1:

The ready biodegradability of the substance was predicted through EPI SUITE (BIOWIN v4.11). The substance was not found to be readily biodegradable based on overall results from several BIOWIN predictions (US EPA, 2018).

Study 2:

A study was conducted to determine the ready biodegradability of the read across substance according to OECD Guideline 301 C. This study was performed in order to evaluate aerobic elimination and degradation potential of the test substance throughout a 39-day period in a test for ready biodegradability, using a nominal test substance concentration of 100 mg/mL in an activated industrial sludge, (adapted model). The degradation was followed by determination of the oxygen consumption. Measurements were taken at the following days: 19, 20, 25, 28 and 39. Based on the results of the read across study, the degree of biodegradation reached 30 - 40 % after 39 d, therefore the test substance is considered to be not readily biodegradable (BASF, 1988).